Mitra S N, Kar S, Ghosh R K, Pajni S, Ghosh A
Indian Institute of Chemical Biology, Jadavpur, Calcutta.
J Med Microbiol. 1995 Jun;42(6):399-403. doi: 10.1099/00222615-42-6-399.
Four outbreak strains of Vibrio cholerae O139 from endemic areas of India and Bangladesh were found to carry lysogenic phage(s). All of these phage(s) produced turbid plaques characteristic of lysogeny on V. cholerae MAK 757 (El Tor, Ogawa) cells as well as on their VcA-1 lysogens but were unable to infect V. cholerae 154 (classical) cells, the universal host for all classical phages. Colonies in the turbid plaques were O139 lysogens and these developed an auxotrophic requirement, mainly for purines suggesting the integration of the prophage into the host chromosome. The immunity profile of the O139 phage(s) was similar to that of phage alpha but differed in the sensitivity of the phage lysogen of V. cholerae MAK 757 to subsequent infection by phage beta.
在印度和孟加拉国的霍乱流行地区发现了四株霍乱弧菌O139的暴发菌株携带溶原性噬菌体。所有这些噬菌体在霍乱弧菌MAK 757（埃尔托生物型，小川型）细胞及其VcA-1溶原菌上产生了溶原性特有的浑浊噬菌斑，但无法感染霍乱弧菌154（古典生物型）细胞，而古典生物型细胞是所有古典噬菌体的通用宿主。浑浊噬菌斑中的菌落是O139溶原菌，这些溶原菌出现了营养缺陷型需求，主要是对嘌呤的需求，这表明原噬菌体整合到了宿主染色体中。O139噬菌体的免疫谱与α噬菌体相似，但在霍乱弧菌MAK 757的噬菌体溶原菌对随后β噬菌体感染的敏感性方面有所不同。
