Rüssmann H, Kothe E, Schmidt H, Franke S, Harmsen D, Caprioli A, Karch H
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Germany.
J Med Microbiol. 1995 Jun;42(6):404-10. doi: 10.1099/00222615-42-6-404.
The pheno- and genotypes of Shiga-like toxins (SLTs) in non-O157 Escherichia coli strains from patients with haemolytic uraemic syndrome were determined. The clinical isolates investigated were from Italy and Germany and belonged to serotypes O22:H8, O26:H-, O26:H11, O91:H-, O111:H- and O128:H-; one isolate was non-typable. SLT genotypes were analysed by complete nucleotide sequence analysis of the B-subunit genes. The results showed that 14 strains possessed slt-I alone, two contained slt-II alone and five isolates harboured both slt-I and slt-II genes. In only two strains were slt-II-related genes found, together with either slt-I or slt-II. These findings indicate that variants of SLT-II are rarely found in non-O157 E. coli isolates from patients with haemolytic uraemic syndrome. Polymerase chain reaction (PCR) with Taq cycle sequencing was found to be a suitable method for classification of slt genotypes.
对溶血尿毒综合征患者的非O157大肠杆菌菌株中志贺样毒素（SLTs）的表型和基因型进行了测定。所研究的临床分离株来自意大利和德国，属于O22:H8、O26:H-、O26:H11、O91:H-、O111:H-和O128:H-血清型；有一株分离株无法分型。通过对B亚基基因进行完整的核苷酸序列分析来分析SLT基因型。结果显示，14株菌株仅携带slt-I，2株仅含有slt-II，5株分离株同时含有slt-I和slt-II基因。仅在2株菌株中发现了与slt-II相关的基因，同时伴有slt-I或slt-II。这些发现表明，在溶血尿毒综合征患者的非O157大肠杆菌分离株中很少发现SLT-II的变体。发现采用Taq循环测序的聚合酶链反应（PCR）是一种适用于slt基因型分类的方法。
