Sartorelli P, Aprea C, Cenni A, Matteucci G, Novelli M T, Sciarra G
Istituto di Medicina del Lavoro, Università degli Studi di Siena.
Med Lav. 1995 Jan-Feb;86(1):34-9.
It is well known that polycyclic aromatic hydrocarbons (PAHs) are absorbed through the skin of experimental animals and humans. However dermal uptake of PAHs is difficult to assess due to the lack of specific studies. The aim of this study was to obtain in vitro percutaneous absorption data for phenanthrene. In vitro penetration was measured with excised monkey (Cercopithecus Aethiops) skin in Franz diffusion cells, using a saline solution with 4% bovine serum albumin and gentamicin sulfate as receptor fluid. In two different groups of 7 and 2 cells, 38.1 and 95.3 nmol/cm2 respectively of phenanthrene were applied in an acetone vehicle. The absorption rate in the first group of 7 cells was 0.025 nmol/cm2/h (S.D. = 0.012), the lag time 11.7 (S.D. = 7.0) h and the breakthrough time 70 m (S.D. = 55 m). In the second group of 2 cells, the average absorption rate was 0.066 nmol/cm2/h and the average lag time 11.8 h. The percutaneous absorption at 24, 48 and 72 hours in the 7 cell group was 0.72% (S.D. = 0.52), 2.51% (S.D. = 1.76) and 3.9% (S.D. = 2.0) of the applied dose respectively. In the 2-cell group 1.1%, 2.78% and 4.5% of the applied dose was absorbed at 24, 48 and 72 hours. The results of the present study confirm the extent of percutaneous absorption of phenanthrene. The data can be compared with dermal absorption values of other PAHs obtained under the same experimental conditions.
众所周知，多环芳烃（PAHs）可通过实验动物和人类的皮肤被吸收。然而，由于缺乏具体研究，PAHs的皮肤吸收情况难以评估。本研究的目的是获取菲的体外经皮吸收数据。使用含4%牛血清白蛋白和硫酸庆大霉素的盐溶液作为接收液，在Franz扩散池中用切除的猴（非洲绿猴）皮肤测量体外渗透率。在两组分别为7个和2个细胞的实验中，将38.1和95.3 nmol/cm²的菲溶于丙酮载体中进行涂抹。第一组7个细胞的吸收速率为0.025 nmol/cm²/h（标准差 = 0.012），滞后时间为11.7（标准差 = 7.0）h，突破时间为70分钟（标准差 = 55分钟）。在第二组2个细胞中，平均吸收速率为0.066 nmol/cm²/h，平均滞后时间为11.8 h。7个细胞组在24、48和72小时的经皮吸收量分别为给药剂量的0.72%（标准差 = 0.52）、2.51%（标准差 = 1.76）和3.9%（标准差 = 2.0）。在2个细胞组中，24、48和72小时的吸收量分别为给药剂量的1.1%、2.78%和4.5%。本研究结果证实了菲的经皮吸收程度。这些数据可与在相同实验条件下获得的其他PAHs的皮肤吸收值进行比较。
