Yang J J, Roy T A, Mackerer C R
Toxicol Ind Health. 1986 Dec;2(4):79-84.
The present study was conducted to evaluate the applicability of in vitro percutaneous absorption techniques to estimate in vivo absorption of polynuclear aromatic (PNA) compounds commonly found in mineral oils. The percutaneous absorption of 14C-labeled anthracene, a three-ring PNA, was compared in a six-day in vivo and in vitro experiment with female Sprague-Dawley rats following a single topical dose (9.3 micrograms/cm2). In vivo absorption was measured by the presence of 14C radioactivity in urine, feces and tissues and in vitro absorption by penetration of radioactivity through excised skin into the receptor fluid of Franz-type diffusion cells. Several modifications of standard diffusion cell techniques were used which are known to enhance the absorption of lipophilic compounds. Anthracene was observed to readily penetrate the skin in both experiments and the total amounts absorbed in vivo and in vitro were seen to gradually coalesce over time. Within six days after application, 52.3 and 55.9% of the administered anthracene was absorbed in vivo and in vitro, respectively.
本研究旨在评估体外经皮吸收技术在估算矿物油中常见的多环芳烃（PNA）化合物体内吸收情况方面的适用性。在一项为期六天的体内和体外实验中，对雌性斯普拉格 - 道利大鼠单次局部给药（9.3微克/平方厘米）后，比较了三环PNA化合物14C标记蒽的经皮吸收情况。通过检测尿液、粪便和组织中14C放射性来测定体内吸收情况，通过放射性穿过离体皮肤渗透到弗兰兹型扩散池的受体液中来测定体外吸收情况。采用了几种已知可增强亲脂性化合物吸收的标准扩散池技术的改进方法。在两个实验中均观察到蒽易于穿透皮肤，且体内和体外吸收的总量随时间逐渐趋于一致。给药后六天内，体内和体外吸收的蒽分别占给药量的52.3%和55.9%。
