Bozzetti C, Camisa R, Nizzoli R, Manotti L, Guazzi A, Naldi N, Mazza S, Nizzoli V, Cocconi G
Department of Medical Oncology, University Hospital, Parma, Italy.
Surg Neurol. 1995 Mar;43(3):230-3; discussion 234. doi: 10.1016/0090-3019(95)80003-y.
The observation that human meningiomas are rich in steroid hormone receptors has led to the hypothesis that their growth may be hormonally dependent. This study aims to correlate the biochemical expression of estrogen (ER) and progesterone receptors (PgR) with their nuclear immunoreactivity in a large series of meningiomas.
The occurrence of ER and PgR in patients with primary untreated meningiomas was studied with a dextrancoated charcoal method (DCC) and the results were compared with those of an immunocytochemical assay (ICA). Progesterone and estrogen receptor determinations were performed on 103 and 99 meningiomas respectively using the DCC assay. Forty-six and 44 of these samples were immunocytochemically evaluated for the presence of PgR and ER respectively.
Of the 46 samples evaluated by both the methods, 89% were found PgR positive by DCC and 70% by ICA. The overall concordance between PgR-DCC and PgR-ICA was 80%. Whereas low concentrations of ER were found in 8/44 samples (18%) assayed by DCC, specific staining was never observed in any of the samples tested by ICA.
Our findings confirm that the majority of meningiomas are devoid of ER and that the biochemical evidence of PgR correlates well with the nuclear localization of progesterone receptors determined by immunocytochemistry.
人类脑膜瘤富含类固醇激素受体这一观察结果引发了其生长可能依赖激素的假说。本研究旨在探讨在一系列大型脑膜瘤中雌激素（ER）和孕激素受体（PgR）的生化表达与其核免疫反应性之间的相关性。
采用葡聚糖包被活性炭法（DCC）研究原发性未经治疗的脑膜瘤患者中ER和PgR的情况，并将结果与免疫细胞化学分析（ICA）的结果进行比较。分别使用DCC分析法对103例和99例脑膜瘤进行孕激素和雌激素受体测定。其中46例和44例样本分别进行了PgR和ER免疫细胞化学评估。
在两种方法评估的46个样本中，DCC法检测到89%的样本PgR呈阳性，ICA法检测到70%呈阳性。PgR-DCC与PgR-ICA之间的总体一致性为80%。DCC法检测的44个样本中有8个（18%）发现低浓度的ER，而ICA检测的任何样本中均未观察到特异性染色。
我们的研究结果证实，大多数脑膜瘤缺乏ER，并且PgR的生化证据与免疫细胞化学测定的孕激素受体核定位密切相关。
