[采用组织化学和生物化学方法测定乳腺癌中的受体状态（雌激素受体和孕激素受体）]

[Receptor status (ER and PgR) determined with histochemical and biochemical methods in breast carcinoma].

作者信息

Simone G, Paradiso A, Cirillo R, Mangia A, Rella G, Wiesel S, Petroni S, De Benedictis G, De Lena M

机构信息

Unità Operativa di Anatomia Patologica, Istituto Oncologico di Bari.

出版信息

Pathologica. 1991 Jul-Aug;83(1086):449-59.

PMID:1724316

Abstract

Recently, a method similar to ER.ICA has been proposed for the progesterone receptor (PgR) using two monoclonal antibodies, JZB39 and KD68, specific for human PgR and characterized by a molecular weight of 95 and 120 Kd, respectively. A series of 73 breast cancer patients was studied with regards to ER and PgR using both immunocytochemical (ICA) and biochemical (DCC) assays. Results showed no substantial differences between the two methods when considering common clinical-pathological parameters. Overall agreement between ICA and DCC methods was found: 79% for PgR and 78% for ER. A slight quantitative correlation was also observed between the "score values" of the ICA method and the Fmol content of ER and PgR using the Brave-Pearson test (r = 0.49 for PgR; r = 0.43 for ER). Specificity of PgR.ICA method was 77% for PgR and 72% for ER; sensitivity was 82% and 83%, respectively. The ICA method is a reliable technique to assess PgR presence as well as ER. Further studies are necessary to evaluate the prognostic role of nuclear PgR.

摘要

最近，一种类似于雌激素受体免疫细胞化学分析（ER.ICA）的方法被用于检测孕激素受体（PgR），该方法使用了两种针对人PgR的单克隆抗体JZB39和KD68，其分子量分别为95 kDa和120 kDa。我们使用免疫细胞化学（ICA）和生化（DCC）分析方法，对73例乳腺癌患者的雌激素受体（ER）和孕激素受体（PgR）进行了研究。在考虑常见临床病理参数时，结果显示两种方法之间没有实质性差异。发现ICA和DCC方法之间的总体一致性：PgR为79%，ER为78%。使用布赖恩-皮尔逊检验（Brave-Pearson test），还观察到ICA方法的“评分值”与ER和PgR的Fmol含量之间存在轻微的定量相关性（PgR的r = 0.49；ER的r = 0.43）。PgR-ICA方法的特异性对于PgR为77%，对于ER为72%；敏感性分别为82%和83%。ICA方法是评估PgR和ER存在情况的可靠技术。有必要进一步研究以评估核PgR的预后作用。