Ono E, Taharaguchi S, Watanabe S, Nikami H, Shimizu Y, Kida H
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Vet Microbiol. 1998 Feb 28;60(2-4):107-17. doi: 10.1016/s0378-1135(97)00153-3.
A mutant form of the immediate-early (IE) protein IE180 of pseudorabies virus (PRV), dIN454-C1081 is a strong repressor of the PRV IE gene promoter. In order to assess the antiviral potential of the IE180 mutant, HeLa cells were transformed with the mutant gene and then infected with PRV and herpes simplex virus type 1 (HSV-1). The transformed cell lines showed marked resistance to PRV infection, but were susceptible to infection with HSV-1, indicating that the IE180 mutant expressed in the stable cell line specifically inhibited PRV growth. In those cells infected with PRV, transcription of the PRV IE gene was repressed. In addition, the IE180 mutant exhibited a dominant-negative property in transient expression assay. The present results indicate that the resistance of the cells to PRV infection was due to repression of the IE gene transcription by the IE 180 mutant.
伪狂犬病病毒（PRV）的立即早期（IE）蛋白IE180的突变形式dIN454-C1081是PRV IE基因启动子的强抑制剂。为了评估IE180突变体的抗病毒潜力，用突变基因转化HeLa细胞，然后用PRV和1型单纯疱疹病毒（HSV-1）感染。转化后的细胞系对PRV感染表现出明显的抗性，但对HSV-1感染敏感，这表明在稳定细胞系中表达的IE180突变体特异性抑制了PRV的生长。在那些感染PRV的细胞中，PRV IE基因的转录受到抑制。此外，IE180突变体在瞬时表达试验中表现出显性负性特性。目前的结果表明，细胞对PRV感染的抗性是由于IE 180突变体对IE基因转录的抑制。
