干扰素-α对伪狂犬病病毒即刻早期基因表达的负调控
Negative regulation of immediate-early gene expression of pseudorabies virus by interferon-alpha.
作者信息
Tonomura N, Ono E, Shimizu Y, Kida H
机构信息
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Japan.
出版信息
Vet Microbiol. 1996 Dec;53(3-4):271-81. doi: 10.1016/s0378-1135(96)01260-6.
Pseudorabies rabies (PrV) replication in Vero cells was suppressed by treatment with human natural interferon-alpha (IFN-alpha). Messenger RNA transcribed from the PrV immediate-early (IE) gene was reduced in the IFN-alpha-treated cells. Transient expression assays showed that transcription from the PrV IE promoter was selectively inhibited in the IFN-alpha-treated cells. Analysis of deletion mutants of the PrV IE promoter sequence suggested that at least one element between the transcription initiation site (+1) and -90 in the PrV IE promoter was concerned with the negative regulation.
人天然α干扰素（IFN-α）处理可抑制伪狂犬病病毒（PrV）在Vero细胞中的复制。在经IFN-α处理的细胞中，PrV立即早期（IE）基因转录的信使RNA减少。瞬时表达分析表明，在经IFN-α处理的细胞中，PrV IE启动子的转录受到选择性抑制。对PrV IE启动子序列缺失突变体的分析表明，PrV IE启动子中转录起始位点（+1）和-90之间至少有一个元件与负调控有关。
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