[The effect of application-sorption therapy on dynamics of activity of molecular carriers of electrons of the energetic and detoxifying systems of cells in experimental burn disease].

Integral free radical state, paramagnetic enzyme complexes of nonheme iron with sulfur- and nitrogen-containing ligands in mitochondria and heme iron in microsomes as well as metalloenzymes of blood plasma ceruloplasmin and transferrin were studied in dynamics of burn disease in rats. Significant decrease in content of reduced ferrosulfur proteins in mitochondrial energy system was observed in kidneys within the second day after the burns and in liver tissue within the 14-21 days after the failure which correlated with reduction of cytochrome P-450 oxidized form. Development of paramagnetic nitrosyl complexes of heme and nonheme iron, specific for hypoxia, were detected in the impaired muscle tissue. Increase in tissue hypoxia was accompanied by decrease in content of transferrin and by elevation in the antioxidant activity of blood plasma. Application of local adsorption after early excision of burns crust contributed to normalization of both metabolism in the impaired tissues and in activity of the electron transport chain in liver and kidney tissues. Therefore, local adsorption therapy may be considered as an alternative procedure in sorptional detoxication of burns.