使用高效液相色谱法测定血浆和食管组织中伊曲康唑水平的简化方法的潜在用途。
Potential use of a simplified method for determination of itraconazole levels in plasma and esophageal tissue by using high-performance liquid chromatography.
作者信息
Darouiche R O, Setoodeh A, Anaissie E J
机构信息
Department of Medicine, Veterans Affairs Medical Center, Houston, TX 77030, USA.
出版信息
Antimicrob Agents Chemother. 1995 Mar;39(3):757-9. doi: 10.1128/AAC.39.3.757.
Abstract
A simplified high-performance liquid chromatography assay was developed for determination of itraconazole levels in plasma and esophageal tissue in four patients with AIDS who had been receiving daily oral doses of 100 mg of itraconazole in solution for at least 3 weeks for therapy of esophageal candidiasis. Itraconazole levels were about three times higher in esophageal tissue than in plasma (means +/- standard errors of 0.69 +/- 0.50 micrograms/g and 0.24 +/- 0.16 micrograms/ml, respectively; P = 0.04). This method is quick (it requires only 1 h for completion) and sensitive (the limits of detectability for itraconazole in plasma and esophageal tissue are 0.005 micrograms/ml and 0.01 micrograms/g, respectively), and it can be reliably used in clinical and research settings (accuracy, > 95%; absolute recovery from biological samples, 80 to 90%; coefficient of variation, 3.3 to 6.6%).
摘要
开发了一种简化的高效液相色谱分析法，用于测定4例艾滋病患者血浆和食管组织中的伊曲康唑水平。这些患者因食管念珠菌病接受每日口服100mg伊曲康唑溶液治疗至少3周。食管组织中的伊曲康唑水平约为血浆中的三倍（分别为0.69±0.50μg/g和0.24±0.16μg/ml的均值±标准误；P = 0.04）。该方法快速（仅需1小时即可完成）且灵敏（血浆和食管组织中伊曲康唑的检测限分别为0.005μg/ml和0.01μg/g），可可靠地用于临床和研究环境（准确度> 95%；生物样品的绝对回收率为80%至90%；变异系数为3.3%至6.6%）。
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