Prevedourakis C, Koumentakou E, Zolotas J, Zolotas T, Xygakis A, Kotoulas I
Acta Obstet Gynecol Scand. 1976;55(3):245-8. doi: 10.3109/00016347609156921.
Amniotic fluid samples were collected aseptically from 29 normal pregnancies, between the 38th and 41st week of gestation and checked for sterility in the laboratory, in order to investigate in vitro the effect of the liquor on the growth of E. coli. Brain Heart Infusion (B.H.I.) and Ringer solution were used as controls. E. coli cultures were inoculated in amniotic fluid, B.H.I., Ringer solution and amniotic fluid plus B.H.I. and incubated at 37degreesC for 48 hours. At 0, 1, 3, 6, 12, 24, 36 and 48 hours of incubation, surface viable counts were performed to estimate the number of E. coli viable cells. The growth of E. coli in B.H.I. started furing the 1st hours after inoculation and continued over 48 hours; in Ringer solution the mean growth curve and almost identical to that of B.H.I. In amniotic fluid the growth of E. coli began from the 1st hour of inoculation but the growth curve was much lower, became static in 24 hours and a permanent inhibition was observed thereafter. The addition of a small amount of B.H.I. in amniotic fluid enhanced the growth of E. coli, but the growth curve was lower in comparison to the curves of the two controls studied. In conclusion, after 48 hours of inoculation in amniotic fluid, 26 of the 29 cases showed bacteriostatic or bactericidal activity of the liquor upon the growth of E. coli.
在妊娠第38至41周期间，从29例正常妊娠中无菌采集羊水样本，并在实验室检查其无菌性，以便在体外研究羊水对大肠杆菌生长的影响。使用脑心浸液（B.H.I.）和林格溶液作为对照。将大肠杆菌培养物接种到羊水、B.H.I.、林格溶液以及羊水加B.H.I.中，并在37℃下孵育48小时。在孵育的0、1、3、6、12、24、36和48小时，进行表面活菌计数以估计大肠杆菌活菌数量。大肠杆菌在B.H.I.中的生长在接种后的第1小时开始，并持续超过48小时；在林格溶液中，平均生长曲线与B.H.I.的几乎相同。在羊水中，大肠杆菌的生长从接种后的第1小时开始，但生长曲线要低得多，在24小时时停止增长，此后观察到永久性抑制。在羊水中添加少量B.H.I.可增强大肠杆菌的生长，但与所研究的两个对照曲线相比，生长曲线较低。总之，在羊水中接种48小时后，29例中的26例显示羊水对大肠杆菌的生长具有抑菌或杀菌活性。
