Yuan G F, Liu C S, Chen C C
Culture Collection and Research Center, Food Industry Researcch and Development Institute, Taiwan, Republic of China.
Appl Environ Microbiol. 1995 Jun;61(6):2384-7. doi: 10.1128/aem.61.6.2384-2387.1995.
Aspergillus parasiticus and Aspergillus sojae are two morphologically similar species belonging to the Aspergillus section flavi. A new method to distinguish the two species, the aflatoxin producer A. parasiticus and the koji mold A. sojae, was developed. Single primers with arbitrary sequences were used to generate random amplified polymorphic DNA (RAPD) markers from strains of these two species. Three decamers, OPA-04, OPB-10, and OPR-01, allowed adequate discrimination between strains of A. parasiticus and A. sojae in RAPD analyses. A. sojae was further separated into group I and group II with the three primers. On the other hand, A. parasiticus was divided into group A and group B when amplified with OPA-04 and OPR-10 primers. The previously misidentified stain CCRC 32423 and the misclassified strain CCRC 30227 were identified as Aspergillus flavus and A. sojae, respectively, on the basis of RAPD patterns and morphological characteristics. We suggest that the RAPD technique is a rapid and reliable tool to distinguish A. parasiticus from A. sojae.
寄生曲霉和酱油曲霉是黄曲霉群中两个形态相似的物种。已开发出一种区分这两个物种的新方法，即区分产黄曲霉毒素的寄生曲霉和酱油曲霉菌。使用具有任意序列的单引物从这两个物种的菌株中生成随机扩增多态性DNA（RAPD）标记。在RAPD分析中，三个十聚体引物OPA-04、OPB-10和OPR-01能够充分区分寄生曲霉和酱油曲霉的菌株。使用这三个引物，酱油曲霉进一步分为I组和II组。另一方面，当用OPA-04和OPR-10引物扩增时，寄生曲霉分为A组和B组。根据RAPD图谱和形态特征，先前误鉴定的菌株CCRC 32423和错误分类的菌株CCRC 30227分别被鉴定为黄曲霉和酱油曲霉。我们认为RAPD技术是区分寄生曲霉和酱油曲霉的一种快速可靠的工具。
