Steinert M, Engelhard H, Flügel M, Wintermeyer E, Hacker J
Institut für Molekulare Infektionsbiologie, University of Würzburg, Germany.
Appl Environ Microbiol. 1995 Jun;61(6):2428-30. doi: 10.1128/aem.61.6.2428-2430.1995.
The lly locus (legiolysin) mediates the browning of the culture medium of Legionella pneumophila in the late stationary growth phase, presumably as a result of synthesis of homogentisic acid. Mutagenesis of the lly gene of the L. pneumophila Philadelphia I derivative JR32 did not affect intracellular replication in the natural host Hartmannella vermiformis. The Lly-negative mutant, however, showed a markedly decreased resistance to ordinary light. The cloned lly gene conferred an increased resistance to light in recombinant L. pneumophila and Escherichia coli K-12, indicating a contribution of the Lly protein to ecological adaptation of Legionella species.
lly基因座（legiolysin）在稳定生长后期介导嗜肺军团菌培养基的褐变，推测这是由于尿黑酸合成所致。嗜肺军团菌费城I衍生物JR32的lly基因突变并不影响其在天然宿主蠕虫状哈特曼氏阿米巴中的细胞内复制。然而，Lly阴性突变体对普通光的抗性明显降低。克隆的lly基因使重组嗜肺军团菌和大肠杆菌K-12对光的抗性增强，表明Lly蛋白对军团菌属的生态适应有贡献。
