Martel R K, Chapco W
Department of Biology, University of Regina, Saskatchewan, Canada.
Biochem Genet. 1995 Feb;33(1-2):1-11. doi: 10.1007/BF00554553.
Restriction fragment analysis of mitochondrial DNA (mtDNA) was used to examine genetic variation and population structure in 13 species of banded-winged grasshoppers (subfamily Oedipodinae). Total DNA of 246 individuals was digested with 11 restriction enzymes and probed with three cloned EcoRI fragments representing the entire mitochondrial genome of Melanoplus sanguinipes. On average, members of this subfamily were five times more variable than those in another subfamily, Melanoplinae, previously examined. This would appear to lend support to Vickery's claim that the time of origin of Nearctic oedipodines is more ancient than that of melanoplines. With respect to population structure, a few different patterns were exhibited. Species such as Camnula pellucida had populations containing a mixture of haplotypes, some widespread and some geographically restricted. In contrast, Chortophaga viridifasciata populations were characterized by unique assemblages of diverse haplotypes. Phylogeographic hypotheses are advanced to account for these observations.
利用线粒体DNA（mtDNA）的限制性片段分析来研究13种带翅蝗虫（斑翅蝗亚科）的遗传变异和种群结构。用11种限制性酶消化246个个体的总DNA，并用代表血黑蝗整个线粒体基因组的三个克隆EcoRI片段进行探测。平均而言，该亚科成员的变异性是之前研究过的另一个亚科——黑蝗亚科成员的五倍。这似乎支持了维克里的观点，即新北区斑翅蝗的起源时间比黑蝗的更古老。关于种群结构，呈现出几种不同的模式。诸如透明牧草蝗等物种的种群包含单倍型的混合，有些单倍型分布广泛，有些则受地理限制。相比之下，绿纹蝗种群的特征是不同单倍型的独特组合。本文提出了系统地理学假说来解释这些观察结果。
