Ioachim-Velogianni E, Tsironi E, Agnantis N, Datseris G, Psilas K
Department of Pathology, University of Ioannina, Greece.
Ger J Ophthalmol. 1995 Mar;4(2):123-9.
The purpose of our study was to investigate the role of immune mechanisms in the pathogenesis of pterygium using an immunohistochemical technique. Our material consisted of 35 surgically excised pterygia and 7 samples of normal conjunctiva obtained from an equal number of patients. HLA-DR antigen expression in epithelial cells, B-cells, suppressor and helper lymphocytes, Langerhans' cells, and monocytes/macrophages were studied immunohistochemically in frozen sections using anti-human HLA-DR, anti-CD22, anti-CD8, anti-CD4, anti-CD1a, and anti-LeuM5 monoclonal antibodies. Aberrant HLA-DR antigen expression in epithelial cells was detected in 30 of 35 cases of pterygium. Epithelial cells in samples of normal conjunctiva were found to be negative in HLA-DR antigen expression. HLA-DR antigen expression in pterygium was found to be closely related to the density of T4 cells and, especially, of CD4 lymphocytes. The present findings suggest that an immunopathologic mechanism plays a role in the pathogenesis of pterygium.
我们研究的目的是采用免疫组织化学技术探讨免疫机制在翼状胬肉发病机制中的作用。我们的材料包括35例手术切除的翼状胬肉以及从相同数量患者获取的7份正常结膜样本。使用抗人HLA - DR、抗CD22、抗CD8、抗CD4、抗CD1a和抗LeuM5单克隆抗体，通过免疫组织化学方法在冰冻切片中研究上皮细胞、B细胞、抑制性和辅助性淋巴细胞、朗汉斯细胞以及单核细胞/巨噬细胞中HLA - DR抗原的表达。在35例翼状胬肉病例中，有30例检测到上皮细胞中存在异常的HLA - DR抗原表达。正常结膜样本中的上皮细胞HLA - DR抗原表达呈阴性。发现翼状胬肉中HLA - DR抗原表达与T4细胞尤其是CD4淋巴细胞的密度密切相关。目前的研究结果表明免疫病理机制在翼状胬肉的发病机制中起作用。
