Department of Ophthalmology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Eye (Lond). 2012 Nov;26(11):1451-8. doi: 10.1038/eye.2012.194. Epub 2012 Sep 14.
To compare lymphangiogenesis in primary versus recurrent pterygium.
Tissues from 88 excised primary and 34 recurrent pterygia were evaluated, and tissues from 7 nasal epibulbar conjunctivae segments were used as controls. The lymph-vascular area (LVA), lymph-microvascular density (LMD), and lymph-vascular luminal diameter (LVL) were examined and compared between the primary and recurrent pterygia. In addition, the expression of VEGF-A and VEGF-C in the primary and recurrent pterygia were determined by ELISA and real-time PCR. The relationships between the mRNA level and LVA, LMD, and LVL were clarified.
Although there was no significant difference in quantification of LVL between primary and recurrent pterygia, the quantification of LVA and LMD in recurrent pterygia dramatically increased in comparison with primary pterygia (both P-values <0.01). Compared with primary pterygia, the VEGF-A and VEGF-C mRNA levels were up-regulated significantly in recurrent pterygia (both P-values <0.05). There was a significant relationship between VEGF-C mRNA and LVA, LMD, and LVL, while VEGF-A mRNA was only closely correlated with LMD in recurrent pterygia.
Lymphangiogenesis develops in recurrent pterygium, for which transient up-regulation of VEGF-C might be responsible.
比较原发性和复发性翼状胬肉中的淋巴管生成。
评估了 88 例原发性和 34 例复发性翼状胬肉切除组织，并将 7 例鼻侧球结膜段作为对照。在原发性和复发性翼状胬肉之间检查和比较了淋巴管面积（LVA）、淋巴管微血管密度（LMD）和淋巴管腔直径（LVL）。此外，通过 ELISA 和实时 PCR 确定原发性和复发性翼状胬肉中 VEGF-A 和 VEGF-C 的表达。阐明了 mRNA 水平与 LVA、LMD 和 LVL 之间的关系。
虽然原发性和复发性翼状胬肉之间的 LVL 定量没有显著差异，但与原发性翼状胬肉相比，复发性翼状胬肉中的 LVA 和 LMD 定量显著增加（均 P 值<0.01）。与原发性翼状胬肉相比，复发性翼状胬肉中的 VEGF-A 和 VEGF-C mRNA 水平显著上调（均 P 值<0.05）。VEGF-C mRNA 与 LVA、LMD 和 LVL 之间存在显著关系，而 VEGF-A mRNA 仅与复发性翼状胬肉中的 LMD 密切相关。
复发性翼状胬肉中存在淋巴管生成，VEGF-C 的短暂上调可能是其原因。
