Withers S G, Aebersold R
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Protein Sci. 1995 Mar;4(3):361-72. doi: 10.1002/pro.5560040302.
Glycosidases play a key role in a number of biological processes and, as such, are of considerable clinical and biotechnological importance. Knowledge of the identifies of catalytically important active site residues is essential for understanding the catalytic mechanism, for enzyme classification, and for targeted bioengineering of glycosidases with altered characteristics. Here we review and discuss traditional strategies and novel approaches based on tandem mass spectrometry for the identification of the key active site residues in glycosidases.
糖苷酶在许多生物过程中发挥着关键作用，因此在临床和生物技术方面具有相当重要的意义。了解催化重要活性位点残基的身份对于理解催化机制、酶分类以及对具有改变特性的糖苷酶进行靶向生物工程至关重要。在这里，我们回顾并讨论基于串联质谱法鉴定糖苷酶关键活性位点残基的传统策略和新方法。
