Fernández A, Ramos J J, Saez T, Sanz M C, Verde M T
Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Vet Res. 1995;26(3):180-4.
Twenty-three male lambs were intoxicated with 2.5 ppm aflatoxins in their feed for a period of 3 weeks. Thirteen lambs were maintained as a control group (0 ppm aflatoxins in their feed). The coagulation profiles were determined from blood samples that were obtained at 0, 7, 14 and 21 d during the intoxication period and at 1, 2, 4 and 8 d of an 8-d clearance period. Aflatoxicosis in the animals was characterized by an increase in prothrombin time (P < 0.01) from d 14 of the intoxication period until the end of the experiment. An increase (P < 0.05) in fibrinogen concentration was detected beginning on d 21, instead of the expected decrease. This was probably due to the inflammation found in the lungs of the intoxicated animals. No difference in activated partial thromboplastin time was found between intoxicated and control animals. These results suggest that there was a significant change in some coagulation factors of the extrinsic pathway in the intoxicated lambs and that prothrombin time determination could be used as an indicator of aflatoxicosis in lambs.
23只雄性羔羊在3周时间内食用了含有2.5 ppm黄曲霉毒素的饲料。13只羔羊作为对照组（饲料中黄曲霉毒素含量为0 ppm）。在中毒期的第0、7、14和21天以及8天清除期的第1、2、4和8天采集血样，测定凝血指标。动物黄曲霉毒素中毒的特征是，从中毒期第14天到实验结束，凝血酶原时间增加（P < 0.01）。从第21天开始，检测到纤维蛋白原浓度增加（P < 0.05），而不是预期的降低。这可能是由于中毒动物肺部出现炎症。中毒动物和对照动物的活化部分凝血活酶时间没有差异。这些结果表明，中毒羔羊的外源性凝血途径的一些凝血因子发生了显著变化，凝血酶原时间测定可作为羔羊黄曲霉毒素中毒的指标。
