Fernández A, Hernández M, Verde M T, Sanz M
Animal Pathology Department, School of Veterinary Medicine, University of Zaragoza, Spain.
Can J Vet Res. 2000 Jan;64(1):53-8.
Twenty-four female lambs were intoxicated with a diet contaminated with 2 ppm aflatoxin for a period of 37 d. Twelve lambs were maintained as the control group. After this period, the lambs were left for 35 d without aflatoxin in their feed. Performance, hematology and clinical immunology were examined in the intoxicated lambs. A non-significant decrease in body weight was observed in the intoxicated lambs during the intoxication period, whereas a significant decrease (P<0.001) in average daily gain was noted on the last day of intoxication and during the clearance period. No significant differences were observed in erythrocyte count, white blood cell count or differential leukocyte count between the groups. Bacteriostatic activity of the serum was lower in the intoxicated lambs, however, there was no effect on serum opsonic activity. Phagocytosis by the neutrophils was higher during the intoxication period and the levels of IgG were elevated in the intoxicated lambs. In vivo cellular immunity was assessed by intradermal injection of phytohemagglutinin; the response was lower during intoxication period. These results indicate that a lowering in the average daily gain was the most sensitive indicator of aflatoxicosis in lambs, and that the immune response was altered, which could render the animals more susceptible to infectious diseases.
24只雌性羔羊用含2 ppm黄曲霉毒素的污染饲料进行为期37天的中毒处理。12只羔羊作为对照组。在此期间后，羔羊在35天内饲料中不再含有黄曲霉毒素。对中毒羔羊的生产性能、血液学和临床免疫学进行了检测。中毒期间中毒羔羊体重有非显著性下降，而在中毒最后一天和清除期平均日增重有显著性下降（P<0.001）。两组之间的红细胞计数、白细胞计数或白细胞分类计数均未观察到显著差异。中毒羔羊血清的抑菌活性较低，然而，对血清调理活性没有影响。中毒期间中性粒细胞的吞噬作用较高，中毒羔羊的IgG水平升高。通过皮内注射植物血凝素评估体内细胞免疫；中毒期间反应较低。这些结果表明，平均日增重下降是羔羊黄曲霉毒素中毒最敏感的指标，并且免疫反应发生了改变，这可能使动物更容易感染传染病。
