采用高效液相色谱-安培检测法测定尿中游离和结合的3-甲氧基-4-羟基苯乙二醇。

Assay of urinary free and conjugated 3-methoxy-4-hydroxyphenylethyleneglycol by high-performance liquid chromatography with amperometric detection.

作者信息

Bourdeaux R, Capolaghi B, Lacroix C, Younos C, Lehr P R

机构信息

Laboratoire de Biochimie, Centre Hospitalier Regional Thionville, France.

出版信息

J Chromatogr B Biomed Appl. 1995 Mar 10;665(1):45-51. doi: 10.1016/0378-4347(94)00516-8.

DOI:10.1016/0378-4347(94)00516-8

PMID:7795800

Abstract

The aim of this study was to develop an analytical method for free and conjugated 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) in urine. After hydrolysis of the conjugated forms, the urinary MHPG was purified by solid-phase extraction on anion exchanger and eluted with a water-methanol (1:1, v/v) mixture. After addition of ethyl acetate to the eluate and back-extraction into acetic acid, the aqueous phase was separated on a C18 column by HPLC and detected amperometrically. The results obtained from forty healthy human subjects were compared with the literature values. The precision and accuracy of the assay were studied using 4-methoxy-3-hydroxyphenylethyleneglycol (iso-MHPG) as internal standard.

摘要

本研究的目的是开发一种分析尿液中游离和结合型3-甲氧基-4-羟基苯乙二醇（MHPG）的方法。结合型形式水解后，尿中的MHPG通过阴离子交换剂进行固相萃取纯化，并用甲醇-水（1:1，v/v）混合物洗脱。向洗脱液中加入乙酸乙酯并反萃取到乙酸中后，水相通过高效液相色谱在C18柱上分离并用安培法检测。将从40名健康人类受试者获得的结果与文献值进行比较。以4-甲氧基-3-羟基苯乙二醇（异-MHPG）为内标研究了该测定法的精密度和准确度。