Brown T J, Shipley L A
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA.
J Chromatogr B Biomed Appl. 1995 Mar 24;665(2):337-44. doi: 10.1016/0378-4347(94)00538-g.
A GC method is described for the determination of xanomeline (LY246708 tartrate) and selected metabolites in rat and monkey plasma. The analytes, including an internal standard, were extracted from plasma at basic pH with hexane. The organic extract was evaporated to dryness and the residue was reconstituted in hexane. The analytes were separated from metabolites and endogenous substances using a DB1701 capillary column. The analytes were detected using nitrogen-phosphorus detection (NPD). The limit of quantitation was determined to be 8 ng/ml, and the response was linear from 8 to 800 ng/ml. The method has been successfully applied to rat and monkey samples pursuant to the development of xanomeline as an agent for the symptomatic treatment of Alzheimer's disease.
描述了一种气相色谱法，用于测定大鼠和猴血浆中的占诺美林（酒石酸LY246708）及选定的代谢物。分析物（包括内标）在碱性pH条件下用己烷从血浆中萃取。有机萃取物蒸发至干，残留物用己烷复溶。使用DB1701毛细管柱将分析物与代谢物及内源性物质分离。采用氮磷检测（NPD）对分析物进行检测。定量限确定为8 ng/ml，响应在8至800 ng/ml范围内呈线性。随着占诺美林作为治疗阿尔茨海默病症状的药物的研发，该方法已成功应用于大鼠和猴样本。
