Pasqui A L, Di Renzo M, Bruni F, Fanetti G, Campoccia G, Auteri A
Department of Clinical Immunology and Allergology, University of Siena, Italy.
Drugs Exp Clin Res. 1995;21(1):17-22.
Imipenem is a new beta-lactam antibiotic endowed with very high antimicrobial activity; it is used in severe infections which often occur in those conditions characterized by impairment of the immune system. The aim of this study was to evaluate the effect of imipenem on some immune functions, both in vitro and in vivo. The authors studied the effect in vitro of three different drug concentrations (15, 30 and 60 mg/l) on polymorphonuclear leucocyte (PMN) phagocytosis and superoxide anion production, as well as on lymphomonocyte proliferative response and cytokine production. Preincubation of PMN with the highest dosages (30 and 60 mg/l) was found to increase phagocytosis evaluated via both cytofluorimeter and chemiluminescence, while no effect was detected on superoxide anion production or on lymphomonocyte tests. In the in vivo study, the authors administered imipenem/cilastatin (1500 mg/day) to 15 elderly and diabetic patients, in whom both PMN functions (phagocytosis and superoxide anion production) and lymphocyte tests (CD3, CD4, CD8, CD19, IL2 and sIL2R serum levels) were studied before and on the 3rd and 7th days of treatment. The drug assimilation did not modify the lymphocyte parameters, whereas it increased PMN superoxide anion production and phagocytosis which were depressed in basal conditions. In the former case, such increase was slight and insignificant, whereas in the latter it was significant.
亚胺培南是一种新型β-内酰胺类抗生素，具有很高的抗菌活性；它用于严重感染，这些感染常发生在免疫系统受损的情况下。本研究的目的是评估亚胺培南在体外和体内对某些免疫功能的影响。作者研究了三种不同药物浓度（15、30和60mg/l）在体外对多形核白细胞（PMN）吞噬作用和超氧阴离子产生的影响，以及对淋巴细胞增殖反应和细胞因子产生的影响。发现用最高剂量（30和60mg/l）预孵育PMN可增加通过细胞荧光仪和化学发光法评估的吞噬作用，而对超氧阴离子产生或淋巴细胞检测未检测到影响。在体内研究中，作者给15名老年糖尿病患者服用亚胺培南/西司他丁（1500mg/天），在治疗前以及治疗第3天和第7天研究了他们的PMN功能（吞噬作用和超氧阴离子产生）和淋巴细胞检测指标（CD3、CD4、CD8、CD19、IL2和sIL2R血清水平）。药物吸收未改变淋巴细胞参数，而增加了在基础条件下降低的PMN超氧阴离子产生和吞噬作用。在前一种情况下，这种增加轻微且无统计学意义，而在后一种情况下则具有统计学意义。
