Dhondt A, Vanholder R, Waterloos M A, Glorieux G, De Smet R, Ringoir S
Dept. of Internal Medicine, University Hospital, Gent, Belgium.
Infection. 1998 Mar-Apr;26(2):120-5. doi: 10.1007/BF02767775.
Phagocytosis is an important part of the host defense against infection. Antibiotics can influence phagocytic function. In the present study, leukocyte metabolic response to phagocytic challenge by latex was assessed in relation to in vitro addition of cotrimoxazole, imipenem/cilastatin, cefodizime, dexamethasone (DXM), and/or cyclosporin A (CsA). Using latex particles as phagocytic challenge, glucose-1-14C utilization and 14CO2 production were measured by liquid scintillation counting. The phagocytic response was impaired by in vitro addition of DXM or CsA and this setup was used as an experimental model of immunodepression. The addition of co-trimoxazole to control samples (without DXM or CsA) depressed the response to latex challenge, whereas imipenem and cefodizime had a neutral effect. In the presence of DXM, co-trimoxazole induced a further decrease. The depressive effect of DXM was partially neutralized in the presence of cefodizime. With CsA depression, co-trimoxazole also induced a further decrease, imipenem had a neutral effect, while cefodizime partially restored the CsA suppressed reaction. Co-trimoxazole depressed the phagocytic response, imipenem had a neutral effect, whereas cefodizime restored the experimentally induced immunosuppression.
吞噬作用是宿主抵御感染的重要组成部分。抗生素可影响吞噬功能。在本研究中，评估了体外添加复方新诺明、亚胺培南/西司他丁、头孢地嗪、地塞米松（DXM）和/或环孢素A（CsA）时，白细胞对乳胶吞噬刺激的代谢反应。以乳胶颗粒作为吞噬刺激物，通过液体闪烁计数法测量葡萄糖-1-14C的利用和14CO2的产生。体外添加DXM或CsA会损害吞噬反应，此设置用作免疫抑制的实验模型。向对照样品（无DXM或CsA）中添加复方新诺明会降低对乳胶刺激的反应，而亚胺培南和头孢地嗪则无影响。在存在DXM的情况下，复方新诺明会导致进一步降低。在存在头孢地嗪的情况下，DXM的抑制作用部分被抵消。对于CsA抑制，复方新诺明也会导致进一步降低，亚胺培南无影响，而头孢地嗪部分恢复了CsA抑制的反应。复方新诺明降低吞噬反应，亚胺培南无影响，而头孢地嗪恢复了实验诱导的免疫抑制。
