Schlein Y, Sira D T, Jacobson R L
Ann Trop Med Parasitol. 1976 Jun;70(2):227-30. doi: 10.1080/00034983.1976.11687116.
Rabbits were immunized with brain, muscle or haemolymph of adult Sarcophaga falculata, and groups of Sarcophaga were then fed on serum collected from the immune rabbits. Rabbit serum immunoglobulins on the tissues of the fed flies were demonstrated by the indirect fluorescent antibody method, using fluorescent goat-anti-rabbit IgG. The antibodies were specifically demonstrated in the tissues that had been used as immunizing antigen. It was also shown that antibodies unrelated to fly antigens pass through the fly gut and react with the specific antigens.
用成年镰刀肉蝇的脑、肌肉或血淋巴对兔子进行免疫,然后让几组肉蝇取食从免疫兔子身上采集的血清。使用荧光山羊抗兔IgG,通过间接荧光抗体法在取食苍蝇的组织上检测兔血清免疫球蛋白。抗体在用作免疫抗原的组织中得到特异性显示。还表明与苍蝇抗原无关的抗体穿过苍蝇肠道并与特异性抗原发生反应。