溃疡性结肠炎固有层细胞产生针对细胞骨架蛋白的免疫球蛋白G及G1抗体。

Production of immunoglobulin G and G1 antibodies to cytoskeletal protein by lamina propria cells in ulcerative colitis.

作者信息

Biancone L, Mandal A, Yang H, Dasgupta T, Paoluzi A O, Marcheggiano A, Paoluzi P, Pallone F, Das K M

机构信息

Cattedra di Gastroenterologia, Universita La Sapienza, Rome, Italy.

出版信息

Gastroenterology. 1995 Jul;109(1):3-12. doi: 10.1016/0016-5085(95)90263-5.

DOI:10.1016/0016-5085(95)90263-5

PMID:7797027

Abstract

BACKGROUND & AIMS: Recent studies suggest an autoantigenic role for tropomyosin-related protein(s) in ulcerative colitis (UC). This study examined whether immunoglobulin G and G1 subclass antibodies against tropomyosins are produced spontaneously by the lamina propria mononuclear cells (LPMCs) that infiltrate the inflamed UC tissue.

METHODS

LPMCs were isolated from colonic biopsy specimens from 29 patients with UC, 15 with colonic Crohn's disease (CD), and 13 with non-inflammatory bowel disease (IBD). The autologous peripheral blood mononuclear cells (PBMCs) were obtained. Cells were cultured in vitro and unstimulated for 10 days. Spontaneous production of immunoglobulin G and G1 antibodies against tropomyosins was measured by enzyme-linked immunosorbent assays using highly enriched tropomyosins from skeletal muscle and colonic mucosa.

RESULTS

The total immunoglobulin G produced by LPMCs from both patients with UC and CD was comparable but higher than from patients with non-IBD (P < 0.05). However, immunoglobulin G antibodies to tropomyosins were higher in patients with UC than in patients with CD (P < 0.04) and non-IBD (P < 0.02). LPMCs from patients with symptomatic UC produced higher immunoglobulin G antibodies to tropomyosins than patients with UC in remission (P < 0.03), symptomatic CD (P < 0.04), and non-IBD (P < 0.02). Immunoglobulin G antibodies to tropomyosins predominantly belonged to immunoglobulin G1 subclass. The autologous PBMCs showed comparable results.

CONCLUSIONS

Immunoglobulin G antibodies predominantly belonging to immunoglobulin G1 subclass and reactive against tropomyosin-related protein(s) are spontaneously produced by LPMCs from the colonic mucosa in patients with UC.

摘要

背景与目的

近期研究提示原肌球蛋白相关蛋白在溃疡性结肠炎（UC）中具有自身抗原作用。本研究检测了浸润于UC炎症组织的固有层单核细胞（LPMC）是否自发产生抗原肌球蛋白的免疫球蛋白G及G1亚类抗体。

方法

从29例UC患者、15例结肠克罗恩病（CD）患者及13例非炎症性肠病（IBD）患者的结肠活检标本中分离LPMC。获取自体外周血单核细胞（PBMC）。细胞在体外培养且未受刺激10天。使用来自骨骼肌和结肠黏膜的高度纯化原肌球蛋白，通过酶联免疫吸附测定法检测抗原肌球蛋白的免疫球蛋白G及G1抗体的自发产生情况。

结果

UC患者和CD患者的LPMC产生的总免疫球蛋白G相当，但高于非IBD患者（P<0.05）。然而，UC患者抗原肌球蛋白的免疫球蛋白G抗体高于CD患者（P<0.04）和非IBD患者（P<0.02）。有症状的UC患者的LPMC产生的抗原肌球蛋白免疫球蛋白G抗体高于缓解期UC患者（P<0.03）、有症状的CD患者（P<0.04）和非IBD患者（P<0.02）。抗原肌球蛋白的免疫球蛋白G抗体主要属于免疫球蛋白G1亚类。自体PBMC显示出类似结果。