Athanasou N A, West L, Sallie B, Puddle B
Department of Pathology, Nuffield Orthopaedic Centre, Oxford, UK.
Histopathology. 1995 Mar;26(3):267-72. doi: 10.1111/j.1365-2559.1995.tb01441.x.
Localized amyloid deposition is known to occur commonly in the articular cartilage of elderly patients. Its pathogenesis is uncertain and it is not known if other cartilage-containing tissues also contain amyloid deposits. Systemic amyloid deposits are known to contain highly sulphated glycosaminoglycans, a major constituent of cartilage. As the composition of articular cartilage glycosaminoglycans is known to change with age, we sought to identify whether localized amyloid deposition in cartilage was glycosaminoglycan-related. We examined specimens of articular cartilage over a wide age range and also examined a variety of cartilaginous tumours and tumour-like lesions for the presence or absence of amyloid deposits. Using mucin histochemistry (alcian blue: MgCl2 critical electrolyte concentration) and immunohistochemistry, we found that highly sulphated glycosaminoglycans (0.9 M and 1 M MgCl2), in particular keratan sulphate, localized to amyloid deposits in both articular cartilage and loose bodies derived from the articular surface. Other cartilaginous lesions (including loose bodies of primary synovial chondromatosis) were negative for amyloid and did not contain highly sulphated glycosaminoglycans. These findings suggest that changes in specific highly sulphated glycosaminoglycans may play a role in localized amyloid deposition in articular cartilage.
已知局部淀粉样沉积常见于老年患者的关节软骨中。其发病机制尚不确定，也不清楚其他含软骨组织是否也含有淀粉样沉积物。已知系统性淀粉样沉积物含有高度硫酸化的糖胺聚糖，这是软骨的主要成分。由于已知关节软骨糖胺聚糖的组成会随年龄变化，我们试图确定软骨中的局部淀粉样沉积是否与糖胺聚糖有关。我们检查了广泛年龄范围内的关节软骨标本，还检查了各种软骨肿瘤和肿瘤样病变中是否存在淀粉样沉积物。使用黏蛋白组织化学（阿尔辛蓝：氯化镁临界电解质浓度）和免疫组织化学方法，我们发现高度硫酸化的糖胺聚糖（0.9 M和1 M氯化镁），尤其是硫酸角质素，定位于关节软骨和源自关节表面的游离体中的淀粉样沉积物。其他软骨病变（包括原发性滑膜软骨瘤病的游离体）淀粉样物质呈阴性，且不含有高度硫酸化的糖胺聚糖。这些发现表明，特定高度硫酸化糖胺聚糖的变化可能在关节软骨局部淀粉样沉积中起作用。
