从细胞培养物中快速检测和分离支原体。
Rapid detection and isolation of mycoplasmas from cell cultures.
作者信息
Tomkins G A, MacGregor A, Pye D, Atkinson M
出版信息
Aust J Exp Biol Med Sci. 1975 Aug;53(4):257-63. doi: 10.1038/icb.1975.28.
PMID:779741
Abstract
An improved medium for the isolation of Mycoplasma species from contaminated cell cultures is reported. A modification of the mycoplasma detection method using the uridine/uracil uptake ratio method is described. Results obtained using this method with mycoplasma contaminated cell cultures and with contaminated cell cultures treated with the antibiotic Lincomycin are presented.
摘要
据报道，一种用于从受污染细胞培养物中分离支原体物种的改良培养基。描述了使用尿苷/尿嘧啶摄取比方法对支原体检测方法的一种改进。展示了使用该方法对受支原体污染的细胞培养物以及用抗生素林可霉素处理的受污染细胞培养物所获得的结果。
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