从反硝化副球菌中分离、测序并诱变编码NAD和谷胱甘肽依赖性甲醛脱氢酶（GD-FALDH）的基因，其中GD-FALDH对甲基营养生长至关重要。
Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.
作者信息
Ras J, Van Ophem P W, Reijnders W N, Van Spanning R J, Duine J A, Stouthamer A H, Harms N
机构信息
Department of Microbiology, Biological Laboratory, Vrije Universiteit, Amsterdam, The Netherlands.
出版信息
J Bacteriol. 1995 Jan;177(1):247-51. doi: 10.1128/jb.177.1.247-251.1995.
Abstract
NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.
摘要
反硝化副球菌的烟酰胺腺嘌呤二核苷酸（NAD）和谷胱甘肽依赖性甲醛脱氢酶（GD-FALDH）已被纯化，为一种相对分子质量为150 kDa的四聚体。编码GD-FALDH的基因（flhA）已被分离、测序，并通过插入卡那霉素抗性基因进行了突变。突变菌株不能在甲醇、甲胺或胆碱上生长，而异养生长不受该突变影响。这一发现表明，反硝化副球菌的GD-FALDH对于甲基营养生长过程中产生的甲醛氧化至关重要。
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Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.从反硝化副球菌中分离、测序并诱变编码NAD和谷胱甘肽依赖性甲醛脱氢酶（GD-FALDH）的基因，其中GD-FALDH对甲基营养生长至关重要。
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