反硝化副球菌的S-甲酰谷胱甘肽水解酶与人类酯酶D同源：甲醛解毒的通用途径？
S-formylglutathione hydrolase of Paracoccus denitrificans is homologous to human esterase D: a universal pathway for formaldehyde detoxification?
作者信息
Harms N, Ras J, Reijnders W N, van Spanning R J, Stouthamer A H
机构信息
Department of Microbial Physiology, Vrije Universiteit, Amsterdam, The Netherlands.
出版信息
J Bacteriol. 1996 Nov;178(21):6296-9. doi: 10.1128/jb.178.21.6296-6299.1996.
Abstract
Downstream of flhA, the Paracoccus denitrificans gene encoding glutathione-dependent formaldehyde dehydrogenase, an open reading frame was identified and called fghA. The gene product of fghA showed appreciable similarity with human esterase D and with the deduced amino acid sequences of open reading frames found in Escherichia coli, Haemophilus influenzae, and Saccharomyces cerevisiae. Mutating fghA strongly reduced S-formylglutathione hydrolase activity. The mutant was unable to grow on methanol and methylamine, indicating that the enzyme is essential for methylotrophic growth. S-Formylglutathione hydrolase appears to be part of a formaldehyde detoxification pathway that is universal in nature.
摘要
在编码谷胱甘肽依赖性甲醛脱氢酶的反硝化副球菌基因flhA的下游，鉴定出一个开放阅读框并将其命名为fghA。fghA的基因产物与人类酯酶D以及在大肠杆菌、流感嗜血杆菌和酿酒酵母中发现的开放阅读框推导的氨基酸序列具有明显的相似性。fghA发生突变会强烈降低S-甲酰谷胱甘肽水解酶的活性。该突变体无法在甲醇和甲胺上生长，这表明该酶对于甲基营养生长至关重要。S-甲酰谷胱甘肽水解酶似乎是自然界中普遍存在的甲醛解毒途径的一部分。
相似文献
1
S-formylglutathione hydrolase of Paracoccus denitrificans is homologous to human esterase D: a universal pathway for formaldehyde detoxification?
J Bacteriol. 1996 Nov;178(21):6296-9. doi: 10.1128/jb.178.21.6296-6299.1996.
2
Two-component system that regulates methanol and formaldehyde oxidation in Paracoccus denitrificans.
J Bacteriol. 2001 Jan;183(2):664-70. doi: 10.1128/JB.183.2.664-670.2001.
3
Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG.
J Biol Chem. 2006 May 19;281(20):14514-22. doi: 10.1074/jbc.M600996200. Epub 2006 Mar 27.
4
Physiological role of S-formylglutathione hydrolase in C(1) metabolism of the methylotrophic yeast Candida boidinii.
Microbiology (Reading). 2003 Aug;149(Pt 8):1971-1979. doi: 10.1099/mic.0.26320-0.
5
Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.
J Bacteriol. 1995 Jan;177(1):247-51. doi: 10.1128/jb.177.1.247-251.1995.
6
Cloning and characterization of an S-formylglutathione hydrolase from Arabidopsis thaliana.
Arch Biochem Biophys. 2002 Mar 15;399(2):232-8. doi: 10.1006/abbi.2002.2772.
7
Isolation and characterization of the moxJ, moxG, moxI, and moxR genes of Paracoccus denitrificans: inactivation of moxJ, moxG, and moxR and the resultant effect on methylotrophic growth.
J Bacteriol. 1991 Nov;173(21):6948-61. doi: 10.1128/jb.173.21.6948-6961.1991.
8
Isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of Paracoccus denitrificans and characterization of the mutant strain.
J Bacteriol. 1991 Nov;173(21):6971-9. doi: 10.1128/jb.173.21.6971-6979.1991.
9
Purificaton and characterization of S-formylglutathione hydrolase from a methanol-utilizing yeast, Kloeckera sp. No. 2201.
Biochim Biophys Acta. 1980 Feb 14;611(2):323-32. doi: 10.1016/0005-2744(80)90068-6.
10
Evidence that S-formylglutathione hydrolase and esterase D polymorphisms are identical.
Hum Genet. 1986 Oct;74(2):176-7. doi: 10.1007/BF00282086.
引用本文的文献
1
Collaborative metabolic curation of an emerging model marine bacterium, Alteromonas macleodii ATCC 27126.
PLoS One. 2025 Apr 24;20(4):e0321141. doi: 10.1371/journal.pone.0321141. eCollection 2025.
2
The Moraxella catarrhalis AdhC-FghA system is important for formaldehyde detoxification and protection against pulmonary clearance.
Med Microbiol Immunol. 2024 Mar 6;213(1):3. doi: 10.1007/s00430-024-00785-0.
3
Genome-scale metabolic model of the versatile bacterium Pd1222.
mSystems. 2024 Feb 20;9(2):e0107723. doi: 10.1128/msystems.01077-23. Epub 2024 Jan 5.
4
Generation of stable integration-free pig induced pluripotent stem cells under chemically defined culture condition.
Cell Prolif. 2023 Nov;56(11):e13487. doi: 10.1111/cpr.13487. Epub 2023 May 15.
5
Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.
Microorganisms. 2022 Jan 20;10(2):220. doi: 10.3390/microorganisms10020220.
6
Molecular dissection of a dedicated formaldehyde dehydrogenase from Mycobacterium smegmatis.
Protein Sci. 2022 Mar;31(3):628-638. doi: 10.1002/pro.4258. Epub 2021 Dec 18.
7
First Genome Description of Isolate Bearing NDM-1 from Blood Culture.
Microorganisms. 2021 Aug 17;9(8):1751. doi: 10.3390/microorganisms9081751.
8
Genetic Context Significantly Influences the Maintenance and Evolution of Degenerate Pathways.
Genome Biol Evol. 2021 Jun 8;13(6). doi: 10.1093/gbe/evab082.
9
Global Transcriptional Response of to Formaldehyde Stress Expands the Role of EfgA and Is Distinct from Antibiotic Translational Inhibition.
Microorganisms. 2021 Feb 10;9(2):347. doi: 10.3390/microorganisms9020347.
10
Applying genome-wide CRISPR to identify known and novel genes and pathways that modulate formaldehyde toxicity.
Chemosphere. 2021 Apr;269:128701. doi: 10.1016/j.chemosphere.2020.128701. Epub 2020 Oct 22.
本文引用的文献
1
Studies on the utilization of nitrate by Micrococcus denitrificans.
J Gen Microbiol. 1962 Oct;29:301-10. doi: 10.1099/00221287-29-2-301.
2
Identification of protein coding regions by database similarity search.
Nat Genet. 1993 Mar;3(3):266-72. doi: 10.1038/ng0393-266.
3
Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice.
Mol Gen Genet. 1993 Jan;236(2-3):341-6. doi: 10.1007/BF00277131.
4
The ftf gene encoding the cell-bound fructosyltransferase of Streptococcus salivarius ATCC 25975 is preceded by an insertion sequence and followed by FUR1 and clpP homologues.
J Gen Microbiol. 1993 May;139(5):913-20. doi: 10.1099/00221287-139-5-913.
5
Cloning and high-level expression of the glutathione-independent formaldehyde dehydrogenase gene from Pseudomonas putida.
J Bacteriol. 1994 May;176(9):2483-91. doi: 10.1128/jb.176.9.2483-2491.1994.
6
Sequence of the gene for a NAD(P)-dependent formaldehyde dehydrogenase (class III alcohol dehydrogenase) from a marine methanotroph Methylobacter marinus A45.
FEMS Microbiol Lett. 1994 Sep 1;121(3):349-55. doi: 10.1111/j.1574-6968.1994.tb07125.x.
7
Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.
J Bacteriol. 1995 Jan;177(1):247-51. doi: 10.1128/jb.177.1.247-251.1995.
8
Cloning, sequence analysis, and expression of the gene encoding formaldehyde dismutase from Pseudomonas putida F61.
Biosci Biotechnol Biochem. 1995 Feb;59(2):197-202. doi: 10.1271/bbb.59.197.
9
A novel formaldehyde oxidation pathway in methylotrophic yeasts: methylformate as a possible intermediate.
FEMS Microbiol Lett. 1995 Apr 1;127(3):229-34. doi: 10.1111/j.1574-6968.1995.tb07478.x.
10
Studies on an enzyme, S-formylglutathione hydrolase, of the dissimilatory pathway of methanol in Candida boidinii.
Biochim Biophys Acta. 1980 Jul 10;614(1):81-91. doi: 10.1016/0005-2744(80)90169-2.
Zotero 插件