Eller M S, Harkness D D, Bhawan J, Gilchrest B A
Department of Dermatology, Boston University School of Medicine, Massachusetts 02118.
J Invest Dermatol. 1994 Dec;103(6):785-90. doi: 10.1111/1523-1747.ep12413037.
The cellular retinoic acid-binding proteins (CRABP I and II) are thought to mediate the effects of retinoic acid on target cells. We have used riboprobes complementary to CRABP I and II mRNAs to study the expression of these messages in normal and abnormal human skin. CRABP II was expressed predominantly in the suprabasal layers of the epidermis, with stronger expression in newborn than in sun-protected adult skin. Interestingly, the epidermis adjacent to or overlying squamous cell or basal cell carcinomas also showed strong expression, whereas the tumor cells were negative, with the exception of more differentiated cells surrounding the "keratin pearls" within squamous cell carcinomas. CRABP II mRNA was also found in the more differentiated cells of the hair follicles, in the outer root sheath. CRABP I message was undetectable in the epidermis or in the dermis of normal skin but was detected in the cells of the papillary dermis surrounding basal and squamous cell carcinomas. These data suggest that increased levels of CRABP II mRNA accompany keratinocyte differentiation in vivo.
细胞视黄酸结合蛋白（CRABP I和II）被认为介导视黄酸对靶细胞的作用。我们使用了与CRABP I和II mRNA互补的核糖探针来研究这些信息分子在正常和异常人类皮肤中的表达。CRABP II主要在表皮的基底层以上表达，新生儿皮肤中的表达比防晒的成人皮肤更强。有趣的是，与鳞状细胞癌或基底细胞癌相邻或覆盖其上的表皮也显示出强表达，而肿瘤细胞呈阴性，但鳞状细胞癌内围绕“角质珠”的分化程度较高的细胞除外。在毛囊的更分化细胞即外根鞘中也发现了CRABP II mRNA。在正常皮肤的表皮或真皮中未检测到CRABP I信息分子，但在围绕基底细胞癌和鳞状细胞癌的乳头层真皮细胞中检测到。这些数据表明，体内角质形成细胞分化伴随着CRABP II mRNA水平的升高。
