Sitzmann J H, Bauer F W, Cunliffe W J, Holland D B, Lemotte P K
Department of Dermatology, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Br J Dermatol. 1995 Aug;133(2):241-8. doi: 10.1111/j.1365-2133.1995.tb02622.x.
The aim of this study was to investigate the effects of 13-cis retinoic acid treatment on cellular retinoic acid binding protein II (CRABP II) mRNA expression in sebaceous follicles from acne patients, using in situ hybridization. Biopsies were taken from uninvolved skin areas in close juxtaposition to inflamed comedos before therapy, and at 2-4 or 14-16 weeks of treatment. Paraffin sections were used for in situ hybridization study with riboprobes transcribed from human CRABP II cDNA. After oral treatment with 13-cis retinoic acid, sebaceous glands were reduced in size and atrophic, and the ratio of sebum-free to fully differentiated (sebum-producing) sebocytes was dramatically increased. The CRABP II expression in the sebaceous gland, and to some extent in infundibular structures, was strongly increased compared with the level of expression in the epidermis. The maximum signal was always found in layers of suprabasal sebocytes lacking lipid droplets, but never in the basal layers. These findings indicate a selective activity of 13-cis retinoic acid on CRABP II mRNA expression in the sebaceous glands of acne patients.
本研究的目的是采用原位杂交技术，研究13-顺式维甲酸治疗对痤疮患者皮脂腺毛囊中细胞视黄酸结合蛋白II（CRABP II）mRNA表达的影响。在治疗前以及治疗2 - 4周或14 - 16周时，从与炎性粉刺紧密相邻的未受累皮肤区域取活检组织。石蜡切片用于与从人CRABP II cDNA转录的核糖探针进行原位杂交研究。口服13-顺式维甲酸后，皮脂腺体积减小且萎缩，无皮脂的皮脂腺细胞与完全分化（产生皮脂）的皮脂腺细胞的比例显著增加。与表皮中的表达水平相比，皮脂腺以及在一定程度上漏斗状结构中的CRABP II表达显著增加。最大信号总是出现在缺乏脂滴的基底上层皮脂腺细胞层中，而从未出现在基底层中。这些发现表明13-顺式维甲酸对痤疮患者皮脂腺中CRABP II mRNA表达具有选择性活性。
