Tondella M L, Quinn F D, Perkins B A
Division of Bacteriology, Adolfo Lutz Institute, São Paulo, Brazil.
J Infect Dis. 1995 Jan;171(1):209-12. doi: 10.1093/infdis/171.1.209.
Brazilian purpuric fever (BPF) is a life-threatening pediatric infection caused by Haemophilus influenzae biogroup aegyptius (Hae), an organism formerly associated with only self-limited purulent conjunctivitis. Strains of Hae causing BPF have a 24-MDa plasmid with a specific AccI restriction pattern designated 3031. This plasmid was thought to code for a virulence factor because it had been detected only among Hae strains isolated from BPF cases or their contacts. From 3 typical BPF cases recently identified in São Paulo State, sterile-site Hae isolates were obtained; these isolates were similar to earlier BPF-associated Hae except they did not possess a 3031 plasmid. HindIII restricted chromosomal DNA from these strains was probed with purified 3031 plasmid DNA under high-stringency conditions. There was no evidence that 3031 plasmid DNA had become chromosomally integrated. It appears that the 3031 plasmid does not code for BPF-specific virulence factors.
巴西紫癜热（BPF）是一种由埃及生物群流感嗜血杆菌（Hae）引起的危及生命的儿科感染，该生物体以前仅与自限性脓性结膜炎有关。引起BPF的Hae菌株有一个24-MDa质粒，其具有特定的AccI限制性图谱，命名为3031。该质粒被认为编码一种毒力因子，因为它仅在从BPF病例或其接触者中分离出的Hae菌株中被检测到。从圣保罗州最近确诊的3例典型BPF病例中，获得了无菌部位的Hae分离株；这些分离株与早期与BPF相关的Hae相似，只是它们不具有3031质粒。在高严格条件下，用纯化的3031质粒DNA探测这些菌株经HindIII酶切的染色体DNA。没有证据表明3031质粒DNA已整合到染色体中。看来3031质粒不编码BPF特异性毒力因子。
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