Grippo A A, Anderson S H, Chapman D A, Henault M A, Killian G J
Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.
J Reprod Fertil. 1994 Sep;102(1):87-93. doi: 10.1530/jrf.0.1020087.
Cholesterol and phospholipid concentrations and phospholipase activity were measured in fluid from cannulae collected from the bovine oviductal isthmus and ampulla at different stages of the oestrous cycle. The cholesterol concentration and cholesterol normalized by protein were significantly (P = 0.03) greater in isthmic oviductal fluid (224.3 +/- 42.7 micrograms ml-1 over all stages) than in ampullary oviductal fluid (164.5 +/- 11.3 micrograms ml-1), and maximal concentrations (284.5 +/- 25.5 micrograms ml-1) were found during the luteal stage (serum progesterone concentration > or = 1.5 ng ml-1). The concentrations of the phospholipids sphingomyelin and lysophosphatidylcholine increased at different stages of the cycle and in different regions. In the ampulla, the concentration of sphingomyelin was significantly (P < 0.05) greater in oviductal fluid collected during the luteal phase (12.1 +/- 2.7% of total phospholipids) than in fluid collected near oestrus and ovulation (7.5 +/- 1.5% and 6.9 +/- 1%, respectively). The concentration of lysophosphatidylcholine was greater (P < 0.01) in ampullary (19.2 +/- 1.6% of total phospholipids) than in isthmic oviductal fluid (9.9 +/- 1.1%) collected near ovulation. The ratio of cholesterol to total phospholipid was highest in oviductal fluid collected from the isthmus during all stages (2.3 micrograms ml-1:% total phospholipid), while the minimal ratio was found in ampullary fluid collected near ovulation (1.5). Phospholipase activity was higher (P = 0.03) in isthmic oviductal fluid (20.4 +/- 3.2% product formed) than in ampullary oviductal fluid (14.6 +/- 1.4%); the lowest activity (12.6 +/- 1.7% product formed) was in fluid collected during the phase of the oestrous cycle immediately before ovulation.(ABSTRACT TRUNCATED AT 250 WORDS)
在发情周期的不同阶段，对从牛输卵管峡部和壶腹部插管采集的液体中的胆固醇、磷脂浓度及磷脂酶活性进行了测定。峡部输卵管液中胆固醇浓度及经蛋白标准化后的胆固醇含量显著高于壶腹部输卵管液（P = 0.03），在所有阶段，峡部输卵管液中胆固醇浓度为224.3±42.7微克/毫升，而壶腹部输卵管液中为164.5±11.3微克/毫升，且在黄体期（血清孕酮浓度≥1.5纳克/毫升）发现最高浓度（284.5±25.5微克/毫升）。鞘磷脂和溶血磷脂酰胆碱等磷脂的浓度在发情周期的不同阶段及不同区域有所增加。在壶腹部，黄体期采集的输卵管液中鞘磷脂浓度显著高于发情期和排卵期附近采集的液体（分别为总磷脂的12.1±2.7%、7.5±1.5%和6.9±1%，P < 0.05）。排卵期附近采集的壶腹部输卵管液中溶血磷脂酰胆碱浓度高于峡部输卵管液（分别为总磷脂的19.2±1.6%和9.9±1.1%，P < 0.01）。在所有阶段，从峡部采集的输卵管液中胆固醇与总磷脂的比值最高（2.3微克/毫升:总磷脂%），而在排卵期附近采集的壶腹部液体中该比值最低（1.5）。峡部输卵管液中的磷脂酶活性高于壶腹部输卵管液（P = 0.03），分别为20.4±3.2%产物生成和14.6±1.4%产物生成；发情周期排卵前阶段采集的液体中活性最低（12.6±1.7%产物生成）。（摘要截取自250字）
