Killian G J, Chapman D A, Kavanaugh J F, Deaver D R, Wiggin H B
Dairy Breeding Research Center, Penn State University, University Park 16802.
J Reprod Fertil. 1989 Jul;86(2):419-26. doi: 10.1530/jrf.0.0860419.
The oviducts of 4 cows were cannulated and oviduct fluid was collected daily from the exteriorized cannulas for a total of 5 oestrous cycles. Daily serum samples were assayed for oestradiol-17 beta and progesterone to monitor the oestrous cycle. Data for each cycle were compared for oviduct fluid collected during the non-luteal phase (serum progesterone less than or equal to 1.5 ng/ml) and the luteal phase (serum progesterone greater than 1.5 ng/ml). During the non-luteal phase oviduct fluid volume was higher and the osmolality was lower than during the luteal phase. Total protein, cholesterol and phospholipid secreted daily was greater during the non-luteal phase. Cholesterol and protein concentrations were generally lower during the non-luteal phase, but phospholipid concentrations were generally higher. About 40% of the phospholipid in oviduct fluid was phosphatidylcholine and lysophosphatidylcholine, while phosphatidylinositol and lysophosphatidylinositol accounted for 20%. The ratio of 1-acyl-phospholipid to diacylphospholipid increased during the non-luteal phase. An increased cholesterol to phospholipid ratio, and a decreased cholesterol to protein ratio in oviduct fluid also were associated with the non-luteal phase. Changes in the lipid composition of oviduct fluid during the oestrous cycle may play a role in the preparation of gametes for fertilization.
对4头奶牛的输卵管进行插管，并在总共5个发情周期内每天从外置插管收集输卵管液。每天检测血清样本中的雌二醇-17β和孕酮，以监测发情周期。比较每个周期在非黄体期（血清孕酮小于或等于1.5 ng/ml）和黄体期（血清孕酮大于1.5 ng/ml）收集的输卵管液数据。在非黄体期，输卵管液体积较高，渗透压低于黄体期。非黄体期每日分泌的总蛋白、胆固醇和磷脂更多。非黄体期胆固醇和蛋白质浓度通常较低，但磷脂浓度通常较高。输卵管液中约40%的磷脂是磷脂酰胆碱和溶血磷脂酰胆碱，而磷脂酰肌醇和溶血磷脂酰肌醇占20%。非黄体期1-酰基磷脂与二酰基磷脂的比例增加。输卵管液中胆固醇与磷脂比例增加以及胆固醇与蛋白质比例降低也与非黄体期有关。发情周期中输卵管液脂质组成的变化可能在配子受精准备过程中起作用。
