Tajima Y, Nagasawa Z, Tanabe I, Kusaba K, Tadano J
Clinical Laboratory, Saga Medical School Hospital, Japan.
Microbiol Immunol. 1994;38(8):639-48. doi: 10.1111/j.1348-0421.1994.tb01834.x.
We have recently found a factor (Factor T) in aged mixtures of tungstate and phosphate which greatly enhances the antibacterial effects of beta-lactams on methicillin-resistant strains of staphylococcal species such as methicillin-resistant Staphylococcus aureus (MRSA), but shows only weak effects on methicillin-susceptible S. aureus and bacterial strains other than staphylococci. Factor T alone did not strongly inhibit cell metabolism and bacterial growth unless an excess amount was added. When Factor T was added to the culture medium beforehand, the growth of MRSA cells was rapidly suppressed just after addition of oxacillin (MPIPC). However, the growth of the cells was inhibited gradually when these two reagents were added in reverse order. For full expression of the enhancing effect, it seemed necessary for cells of MRSA strains to be incubated with Factor T for at least 2-3 hr. When the cells were washed after being sensitized by incubating them for 5 hr with Factor T, it took approximately 1 hr for the cells to recover their resistance to MPIPC. Factor T reduced the amount of penicillin-binding protein-2' (PBP 2'), and thus sensitized the MRSA strains to beta-lactams.
我们最近在钨酸盐和磷酸盐的老化混合物中发现了一种因子（因子T），它能极大地增强β-内酰胺类药物对耐甲氧西林葡萄球菌菌株（如耐甲氧西林金黄色葡萄球菌，MRSA）的抗菌作用，但对甲氧西林敏感的金黄色葡萄球菌和葡萄球菌以外的细菌菌株作用较弱。单独的因子T不会强烈抑制细胞代谢和细菌生长，除非添加过量。预先将因子T添加到培养基中，在加入奥沙西林（MPIPC）后，MRSA细胞的生长会立即迅速受到抑制。然而，当以相反顺序添加这两种试剂时，细胞的生长会逐渐受到抑制。为了充分表达增强效果，MRSA菌株的细胞似乎有必要与因子T孵育至少2 - 3小时。当细胞在与因子T孵育5小时致敏后进行洗涤时，细胞大约需要1小时才能恢复对MPIPC的抗性。因子T减少了青霉素结合蛋白2'（PBP 2'）的量，从而使MRSA菌株对β-内酰胺类药物敏感。
