Tajima Y, Nagasawa Z, Tadano J
Clinical Laboratory, Saga Medical School Hospital, Japan.
Microbiol Immunol. 1993;37(9):695-703. doi: 10.1111/j.1348-0421.1993.tb01694.x.
We have found a factor (Factor T) in aged mixtures of tungstate and phosphate which greatly enhances the antibacterial effects of beta-lactams on both inducible and constitutive methicillin-resistant Staphylococcus aureus, but not on methicillin-susceptible S. aureus. Factor T alone did not strongly inhibit bacterial growth. There was no synergism of Factor T with other classes of antibiotics, nor with other groups of bacteria, and it reduced the efficacy of amino-glycosides and tetracycline. Upon preparation of Factor T, acidifying and heating the mixture of tungstate and phosphate resulted in a high yield and reproducibility, and no enhancing effect was observed when other anions such as sulfate or molybdate were used instead. Factor T is heat- and acid-stable but labile to alkalization, and is probably a complex of phosphate and tungstate.
我们在钨酸盐和磷酸盐的老化混合物中发现了一种因子（因子T），它能极大地增强β-内酰胺类药物对诱导型和组成型耐甲氧西林金黄色葡萄球菌的抗菌作用，但对甲氧西林敏感的金黄色葡萄球菌无效。单独的因子T不会强烈抑制细菌生长。因子T与其他类抗生素以及其他细菌群体之间没有协同作用，并且它会降低氨基糖苷类药物和四环素的疗效。在制备因子T时，将钨酸盐和磷酸盐的混合物酸化并加热可获得高产率和可重复性，而使用其他阴离子（如硫酸盐或钼酸盐）代替时则未观察到增强作用。因子T耐热且耐酸，但对碱化不稳定，可能是磷酸盐和钨酸盐的复合物。
