Hilbink F, West D M, de Lisle G W, Kittelberger R, Hosie B D, Hutton J, Cooke M M, Penrose M
Central Animal Health Laboratory, Ministry of Agriculture and Fisheries, Upper Hutt, New Zealand.
Vet Microbiol. 1994 Jul;41(1-2):107-16. doi: 10.1016/0378-1135(94)90140-6.
A complement fixation test for paratuberculosis, a gel diffusion test and two enzyme-linked immunosorbent assays (ELISA) were evaluated using sera from Mycobacterium paratuberculosis infected and non-infected sheep. Gross pathology and histopathology were used as parameters of infection. The two ELISAs, one of which is commercially available for testing cattle, were used before and after sera had been absorbed with a soluble sonicate of Mycobacterium phlei. Differences between the various tests and between ELISAs before and after absorption were non-significant (P > 0.05) in non-infected sheep or in animals with gross or histopathological lesions. The specificity of all the tests was at least 97%. Sensitivity in histopathologically positive sheep was at least 98%. Sheep from infected flocks but without histopathological lesions showed serological results which were poorly correlated between the various tests.
使用来自副结核分枝杆菌感染和未感染绵羊的血清，对副结核病补体结合试验、凝胶扩散试验和两种酶联免疫吸附测定（ELISA）进行了评估。大体病理学和组织病理学用作感染参数。两种ELISA，其中一种可用于牛检测的商业化产品，在血清用草分枝杆菌可溶性超声破碎物吸收前后使用。在未感染绵羊或有大体或组织病理学病变的动物中，各种试验之间以及吸收前后ELISA之间的差异无统计学意义（P>0.05）。所有试验的特异性至少为97%。组织病理学阳性绵羊的敏感性至少为98%。来自感染羊群但无组织病理学病变的绵羊，其血清学结果在各种试验之间相关性较差。
