用单克隆抗体揭示的多杀性巴氏杆菌的血清型和属特异性蛋白质抗原。
Serotype and genus specific protein antigens of Pasteurella multocida, revealed by monoclonal antibodies.
作者信息
Adler B
机构信息
Department of Microbiology, Monash University, Melbourne, Vic., Australia.
出版信息
Vet Microbiol. 1994 Jul;41(1-2):29-40. doi: 10.1016/0378-1135(94)90133-3.
Two monoclonal antibodies (mAbs) reacting with protein antigens of Pasteurella multocida strain M1404 were produced and designated DM-1 and DM-4. DM-1 reacted only with the homologous Heddleston serotype 2. On the other hand, DM-4 reacted with all the 16 Heddleston serotypes and with other Pasteurella spp., but not with any of the other genera tested. Both mAbs may thus be of significance for identification and classification. The antibodies could agglutinate P. multocida only if the cells were treated with 1N HCl. Neither of the mAbs could opsonise P. multocida for phagocytosis by mouse macrophages nor did they protect recipient mice against lethal infection. Active immunisation of mice gave rise to only partial protection against challenge infection, suggesting a minor role for these particular proteins in protection from infection.
制备了两种与多杀性巴氏杆菌M1404株蛋白质抗原发生反应的单克隆抗体（mAb），分别命名为DM-1和DM-4。DM-1仅与同源的赫德尔斯顿2型血清型发生反应。另一方面，DM-4与所有16种赫德尔斯顿血清型以及其他巴氏杆菌属发生反应，但与所检测的其他任何属均无反应。因此，这两种单克隆抗体对于鉴定和分类可能具有重要意义。只有在用1N盐酸处理细胞后，这些抗体才能凝集多杀性巴氏杆菌。这两种单克隆抗体均不能调理多杀性巴氏杆菌以供小鼠巨噬细胞吞噬，也不能保护受体小鼠免受致死性感染。对小鼠进行主动免疫仅能提供部分针对攻击感染的保护，这表明这些特定蛋白质在预防感染中起次要作用。
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