Verma R D, Venkateswaran K S, Sharma J K, Agarwal G S
Central Military Veterinary Laboratory, Meerut Cantt., India.
Vet Microbiol. 1994 Aug 15;41(4):391-7. doi: 10.1016/0378-1135(94)90035-3.
Malleo-proteins from synthetic broth mallein of six strains of Pseudomonas mallei were separated by trichloroacetic acid precipitation, ammonium sulfate precipitation and Ultrogel AcA 34 gel filtration chromatography. When tested comparatively with Dutch PPD mallein as standard on P. mallei-sensitized and normal horses all the strains were found to be malleinogenic, trichloroacetic acid precipitated proteins were comparable to Dutch PPD mallein in potency and innocuity whereas ammonium sulfate-precipitated proteins elicited non-specific reactions. Ultrogel AcA 34 chromatographed high molecular weight proteins (MW range > 350,000) were having equal or higher potency and without adverse reactions and low molecular weight proteins (MW range 120,000-170,000) nearly inactive. Ultrogel AcA 34 column fractionation studies revealed that mallein activity was associated with high molecular weight proteins and represented a purer sensitin than PPD mallein for the purpose of mallein test.
通过三氯乙酸沉淀、硫酸铵沉淀和Ultrogel AcA 34凝胶过滤色谱法，对6株鼻疽假单胞菌合成肉汤鼻疽菌素中的锤骨蛋白进行了分离。当以荷兰PPD鼻疽菌素为标准，对感染鼻疽假单胞菌的马匹和正常马匹进行比较测试时，发现所有菌株都具有产生鼻疽菌素的能力，三氯乙酸沉淀的蛋白质在效力和无害性方面与荷兰PPD鼻疽菌素相当，而硫酸铵沉淀的蛋白质会引发非特异性反应。Ultrogel AcA 34色谱分离的高分子量蛋白质（分子量范围>350,000）具有同等或更高的效力且无不良反应，低分子量蛋白质（分子量范围120,000 - 170,000）几乎没有活性。Ultrogel AcA 34柱分级分离研究表明，鼻疽菌素活性与高分子量蛋白质相关，并且就鼻疽菌素试验而言，它代表了一种比PPD鼻疽菌素更纯的敏感素。
