针对低发生率红细胞抗原Wra的鼠单克隆抗体的制备：特性鉴定及与人类抗Wra抗体的比较

Production of a murine monoclonal antibody to the low-incidence red cell antigen Wra: characterisation and comparison with human anti-Wra.

作者信息

Ring S M, Green C A, Swallow D M, Tippett P

机构信息

MRC Blood Group Unit, University College London, UK.

出版信息

Vox Sang. 1994;67(2):222-5. doi: 10.1111/j.1423-0410.1994.tb01664.x.

DOI:10.1111/j.1423-0410.1994.tb01664.x

PMID:7801616

Abstract

The production of a monoclonal antibody which detects the low-incidence red cell antigen Wra is described. The antibody (BGU1-WR) was isolated following immunization of BALB/tk mice with Wr(a+) cells. BGU1-WR is an IgG1 antibody and reacted in a manner similar to human polyclonal anti-Wra with untreated, protease treated and chemically modified Wr(a+) cells.

摘要

本文描述了一种可检测低发生率红细胞抗原Wra的单克隆抗体的制备。在用Wr(a+)细胞免疫BALB/tk小鼠后，分离出了该抗体（BGU1-WR）。BGU1-WR是一种IgG1抗体，其与未处理的、经蛋白酶处理的以及化学修饰的Wr(a+)细胞反应的方式，类似于人多克隆抗Wra抗体。