Wu J, Yu X J, Ma X, Li X G, Liu D
Electrophysiology Laboratory, Xi-an Medical University, China.
Zhongguo Yao Li Xue Bao. 1994 Jul;15(4):341-3.
The effects of total flavones of Hippophae rhamnoides L (TFH) were evaluated using conventional microelectrode technic. After administration of TFH 100-200 mg.L-1, the action potential duration of 50% repolarization (APD50) was shortened both in cultured rat myocardial cells and in guinea pig papillary muscles. The slope of phase 4 of depolarization (SP4) in cultured rat myocardial cells was decreased and the contractile force (CF) in guinea pig papillary muscles was weakened. Arrhythmias evoked by strophantin G in guinea pig papillary muscles were suppressed by TFH 100 mg.L-1. These findings suggested that the influence of TFH on myocardial cells may be resulted mainly from its inhibition of Ca2+ influx and its interference with intracellular Ca2+ reservoir.
采用传统微电极技术评估沙棘总黄酮（TFH）的作用。给予100 - 200mg.L-1的TFH后，培养的大鼠心肌细胞和豚鼠乳头肌的50%复极化动作电位时程（APD50）均缩短。培养的大鼠心肌细胞去极化第4相斜率（SP4）降低，豚鼠乳头肌收缩力（CF）减弱。100mg.L-1的TFH可抑制毒毛花苷G诱发的豚鼠乳头肌心律失常。这些结果提示，TFH对心肌细胞的影响可能主要源于其对Ca2+内流的抑制及其对细胞内Ca2+储备的干扰。
