Ogura K, Nishiyama T, Hiratsuka A, Watabe T, Watabe T
Department of Drug Metabolism and Molecular Toxicology, Tokyo College of Pharmacy, Japan.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1250-6. doi: 10.1006/bbrc.1994.2799.
The rat gene encoding the class theta glutathione S-transferase (GST) subunit Yrs has been isolated from a rat lambda EMBL4 DNA library and completely sequenced. The gene spans approximately 4 kb comprising 5 exons separated by 4 introns, the smallest number of the gene components among known rat genes encoding GST subunits. Sequence analysis of the 5' flanking region of the GST Yrs gene indicated the absence of both TATA and CAAT boxes. However, potential binding sites for the transcription factors SP1, PU1, PEA3, and AP-2 were present in this region. Primer extension and 5'-rapid amplification of cDNA ends studies demonstrated the existence of multiple transcription initiation sites distributed over 58 bp in the upstream sequence from the translation initiation codon. The present study provides the first evidence for the primary structure of the gene encoding a class theta GST subunit.
编码θ类谷胱甘肽S-转移酶（GST）亚基Yrs的大鼠基因已从大鼠λEMBL4 DNA文库中分离出来并进行了全序列测定。该基因跨度约4kb，由5个外显子和4个内含子组成，在已知的编码GST亚基的大鼠基因中，其基因组成部分数量最少。对GST Yrs基因5'侧翼区域的序列分析表明，既没有TATA盒也没有CAAT盒。然而，该区域存在转录因子SP1、PU1、PEA3和AP-2的潜在结合位点。引物延伸和cDNA末端的5'-快速扩增研究表明，在翻译起始密码子上游序列中，多个转录起始位点分布在58bp范围内。本研究为编码θ类GST亚基的基因的一级结构提供了首个证据。
