[Effect of sexual hormones on proliferation of laryngeal carcinoma cell line HEp-2 in vitro].

In this study, we observed the effect of FCS and DCC FCS on the proliferation of HEp-2 cell in vitro with dextran-coated charcoal technique, and explored the dose-dependence of HEp-2 cell to testosterone, 17 beta-estradiol and progesterone. The results showed that HEp-2 cell line is androgen-sensitive cell, and testosterone at concentration of 1 nmol/L and 10 nmol/L may stimulate the proliferation of HEp-2 cells, but 17 beta-estradiol and progesterone have no such effect. The results suggested that HEp-2 cell line is a proper model in vitro for the study of laryngeal carcinoma and sexual hormones. This study offered the evidences of the endocrine therapy for laryngeal carcinoma.