Le Van Kim C, Mouro I, Brossard Y, Chavinié J, Cartron J P, Colin Y
INSERM U.76, Institut National de la Transfusion Sanguine, Paris, France.
Br J Haematol. 1994 Sep;88(1):193-5. doi: 10.1111/j.1365-2141.1994.tb04996.x.
After anti-RhD, anti-Rhc is the most important red cell alloantibody which can cause haemolytic disease of the newborn (HDN) when the mother is Rhc-negative and the fetus Rhc-positive. We report here the development of polymerase chain reaction (PCR) assays which detect the presence of the Rhc alleles in amniotic cells by the use of allele-specific primers (ASP). It is expected that such determination will help in the management of pregnancies at risk of Rhc haemolytic disease. In the course of this study we have similarly performed PCR-ASP experiments to detect fetal RHE alleles since, in rare cases, anti-RhE can also cause HDN.
除了抗 RhD 抗体外，抗 Rhc 是最重要的红细胞同种抗体，当母亲为 Rhc 阴性而胎儿为 Rhc 阳性时，它可导致新生儿溶血病（HDN）。我们在此报告聚合酶链反应（PCR）检测方法的开发，该方法通过使用等位基因特异性引物（ASP）来检测羊水中 Rhc 等位基因的存在。预计这种检测将有助于对有 Rhc 溶血病风险的妊娠进行管理。在本研究过程中，我们同样进行了 PCR-ASP 实验以检测胎儿的 RHE 等位基因，因为在罕见情况下，抗 RhE 也可导致 HDN。
