Yohn J J, Smith C, Stevens T, Morelli J G, Shurnas L R, Walchak S J, Hoffman T A, Kelley K K, Escobedo-Morse A, Yanagisawa M
Department of Dermatology, University of Colorado School of Medicine, Denver 80262.
Biochim Biophys Acta. 1994 Dec 30;1224(3):454-8. doi: 10.1016/0167-4889(94)90282-8.
We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.
我们研究了内皮素-1（ET-1）受体在正常人角质形成细胞（HK）上的表达。我们发现HK表达ETB受体亚型，并对ET-1作出反应，细胞内游离钙增加2.7倍。HK对ET-1刺激未出现增殖增加；然而，30 nM ET-1可使HK条件培养基中ET-1的积累减少51.7%。我们认为HK的ET-1受体在ET-1分泌的自分泌调节中发挥作用。
