Charnock-Jones D S, Sharkey A M, Boocock C A, Ahmed A, Plevin R, Ferrara N, Smith S K
Department of Obstetrics and Gynaecology, University of Cambridge, Rosie Maternity Hospital, England.
Biol Reprod. 1994 Sep;51(3):524-30. doi: 10.1095/biolreprod51.3.524.
Vascular endothelial growth factor (VEGF; also known as vascular permeability factor) is a secreted angiogenic growth factor. It is highly specific for endothelial cells, and its receptor, the fms-like tyrosine kinase (flt), has been localized only to endothelial cells in vivo. Here we describe the expression of mRNA encoding flt in human trophoblast as revealed by in situ hybridization. This mRNA is highly expressed in the cytotrophoblast shell and columns and also highly expressed by the extravillous trophoblast (EVT) in the maternal decidua both in the first trimester and at term. The trophoblast-like choriocarcinoma cell line BeWo also expresses this receptor and the related receptor, kinase domain-containing receptor (KDR), which is also a receptor for VEGF. Treatment of the cell line BeWo with VEGF165 stimulated 3H-thymidine incorporation and tyrosine phosphorylation of MAP (mitogen-activated protein) kinase in a time- and dose-dependent fashion. This study is the first demonstration of the presence of flt on non-endothelial cells in vivo and suggests a role for VEGF in the growth and differentiation of cytotrophoblast at implantation.
血管内皮生长因子（VEGF；也称为血管通透因子）是一种分泌型血管生成生长因子。它对内皮细胞具有高度特异性，其受体，即fms样酪氨酸激酶（flt），在体内仅定位于内皮细胞。在此，我们描述了通过原位杂交揭示的人滋养层细胞中编码flt的mRNA的表达情况。这种mRNA在细胞滋养层壳和柱中高度表达，并且在孕早期和足月时，在母体蜕膜中的绒毛外滋养层（EVT）中也高度表达。滋养层样绒毛膜癌细胞系BeWo也表达这种受体以及相关受体，即含激酶结构域受体（KDR），它也是VEGF的一种受体。用VEGF165处理细胞系BeWo以时间和剂量依赖的方式刺激了3H-胸腺嘧啶核苷掺入以及丝裂原活化蛋白（MAP）激酶的酪氨酸磷酸化。本研究首次证明了体内非内皮细胞上存在flt，并提示VEGF在着床时细胞滋养层的生长和分化中发挥作用。
