编码新大核草履虫β-端粒结合蛋白的微核基因。

The micronuclear gene encoding beta-telomere binding protein in Oxytricha nova.

作者信息

Mitcham J L, Prescott D M, Miller M K

机构信息

Immunex Research and Development Corporation, Seattle, Washington 98101.

出版信息

J Eukaryot Microbiol. 1994 Sep-Oct;41(5):478-80. doi: 10.1111/j.1550-7408.1994.tb06045.x.

DOI:10.1111/j.1550-7408.1994.tb06045.x

PMID:7804248

Abstract

The micronuclear version of the gene encoding beta-telomere binding protein (beta-TBP) in Oxytricha nova has been sequenced and compared to the macronuclear beta-TBP gene, previously described. The micronuclear gene contains three AT-rich internal eliminated sequences (IES) of 37, 40, and 43 bp and four macronuclear destined sequences (MDS). The IES interrupt the gene once near the 5' end of the coding region and twice in the 3' trailer downstream from the TGA stop codon. The sequences of the micronuclear and macronuclear genes are colinear. Thus, the micronuclear beta-TBP gene is not scrambled, which contrasts with the highly scrambled state among the 14 MDS in the micronuclear alpha-TBP gene.

摘要

已对新大草履虫中编码β-端粒结合蛋白（β-TBP）的基因的微核版本进行了测序，并与先前描述的大核β-TBP基因进行了比较。微核基因包含三个富含AT的内部消除序列（IES），长度分别为37、40和43 bp，以及四个大核定向序列（MDS）。IES在编码区5'端附近中断基因一次，在TGA终止密码子下游的3'尾区中断两次。微核基因和大核基因的序列是共线的。因此，微核β-TBP基因没有发生重排，这与微核α-TBP基因中14个MDS之间的高度重排状态形成对比。