Prescott D M, Greslin A F
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
Dev Genet. 1992;13(1):66-74. doi: 10.1002/dvg.1020130111.
In the hypotrichous ciliate Oxytricha nova the cloned precursor gene from the micronuclear genome that encodes actin I is composed of highly disordered blocks of deoxynucleotide sequences. We present and illustrate in detail a recombination model that explains how the actin I gene may be unscrambled during macronuclear development after cell mating. The model was described in a previous publication (Greslin et al.: Proc Natl Acad Sci USA 86:6264-6268, 1989). Here we show the data, described in the earlier publication, that support the model. The data show that scrambling is not an artifact of cloning. They rule against the presence of an unscrambled copy of the actin I gene in the micronucleus, which means that unscrambling must be a part of macronuclear development. Finally, the data prove that the actin I gene in O. trifallax is scrambled in a pattern that resembles the pattern in O. nova.
在寡毛纲纤毛虫新奥克特里查中，来自微核基因组的编码肌动蛋白I的克隆前体基因由高度无序的脱氧核苷酸序列块组成。我们详细展示并阐释了一个重组模型，该模型解释了在细胞交配后的大核发育过程中肌动蛋白I基因是如何解旋的。该模型在之前的一篇出版物中已有描述（Greslin等人：《美国国家科学院院刊》86:6264 - 6268，1989年）。在此我们展示了早期出版物中支持该模型的数据。这些数据表明解旋并非克隆的假象。它们排除了微核中存在未解旋的肌动蛋白I基因拷贝的可能性，这意味着解旋必定是大核发育的一部分。最后，数据证明三裂奥克特里查中的肌动蛋白I基因是以一种类似于新奥克特里查中的模式解旋的。
