Kétyi I
Institute of Microbiology, University Medical School, Pécs, Hungary.
Acta Microbiol Immunol Hung. 1994;41(2):189-95.
The effectiveness of antibiotics was tested on the autochthonous Escherichia coli in biofilm mode of growth in large bowel pieces as well as on the predominant faecal E. coli isolated from the same SPF mice in planktonic phase of growth. Aminoglycoside antibiotics, chloramphenicol, oxytetracycline, erythromycin and lincomycin-clindamycin treatment had a very limited effect in the intestinal biofilm. Surprising ineffectiveness was found with polymyxins: polymyxin B showed a Minimal Bactericidal Dose of 0.78 microgram in planktonic phase, while it was 400 micrograms for E. coli incorporated in the biofilm matrix. In contrast to the above groups of antibiotics, the beta-lactam drugs were effective both in the biofilm and in the planktonic phase growth of E. coli and their derivatives with broad or broader spectrum exerted an increased biofilm activity. Polymyxin B showed no sign of penetration into the colonic mucus, but on the other side ampicillin concentrated about three-four times in the intestinal matrix.
在大肠组织块中以生物膜生长模式对本地大肠杆菌进行了抗生素有效性测试，同时也对从相同无特定病原体（SPF）小鼠中分离出的处于浮游生长阶段的主要粪便大肠杆菌进行了测试。氨基糖苷类抗生素、氯霉素、土霉素、红霉素和林可霉素 - 克林霉素治疗对肠道生物膜的效果非常有限。多粘菌素显示出惊人的无效性：多粘菌素B在浮游阶段的最小杀菌剂量为0.78微克，而对于生物膜基质中的大肠杆菌，该剂量为400微克。与上述抗生素组不同，β - 内酰胺类药物在大肠杆菌及其衍生物的生物膜和浮游阶段生长中均有效，且具有更广泛或更宽谱的药物表现出增强的生物膜活性。多粘菌素B没有渗透到结肠黏液的迹象，但另一方面，氨苄西林在肠道基质中的浓度约为其三到四倍。
