Kétyi I
Pécsi Orvostudományi Egyetem, Mikrobiológiai Intézete.
Orv Hetil. 1993 Feb 20;134(8):405-8.
Representatives of different groups of antibiotics were tested on the autochtonous Escherichia coli of mice being in biofilm mode of growth, as well as on their isolates in planktonic phase. Aminoglycoside antibiotics, chloramphenicol, oxytetracycline, erythromycin, lincomycin-clindamycin, and polymyxins showed a very reduced effectivity on Escherichia coli embedded in the biofilm matrix. On the other hand, beta-lactam antibiotics were equally effective both for sessile and planktonic bacteria. Derivates with broader, or broad spectra showed an increased biofilm activity. Testing the possibilities of penetration or concentration on mouse colonic pieces exposed to 2 Minimal Bactericidal Doses--polymyxin B showed no sign of penetration into the colonic mucus, while 2.9% of streptomycin and 60% of the exposed quantity of carbenicillin was detected in the intestinal mucus. Ampicillin, however, has concentrated about three-four times in the intestinal matrix.
对处于生物膜生长模式的小鼠体内的本地大肠杆菌及其浮游期分离菌进行了不同组抗生素的测试。氨基糖苷类抗生素、氯霉素、土霉素、红霉素、林可霉素 - 克林霉素和多粘菌素对嵌入生物膜基质中的大肠杆菌的有效性非常低。另一方面，β - 内酰胺类抗生素对固着菌和浮游菌同样有效。具有更广谱或广谱的衍生物表现出更高的生物膜活性。测试了在暴露于2个最小杀菌剂量的小鼠结肠片段上的渗透或浓度可能性——多粘菌素B没有渗透到结肠黏液的迹象，而在肠道黏液中检测到2.9%的链霉素和60%的暴露量的羧苄青霉素。然而，氨苄西林在肠道基质中浓缩了约三四倍。
